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放射测量与光谱测量之间的比较,用于确定脑脊液中血管酶转化酶活性

Ludmia Taibi¹, Bénédicte Bénéteau-Burnat¹, Michel Vaubourdolle¹

¹Biochimie, Hôpital Saint-Antoine, DMU BioGem, Sorbonne University, 184 rue du faubourg Saint-Antoine, 75571 Paris cedex 12, France.

Annales de biologie clinique

|June 17, 2023

概括

在脑脊液 (CSF) 中评估血管素转化酶 (ACE) 活性有助于诊断神经类症. 放射测量和光谱测试与ELISA进行了比较,放射测量显示CSF中ACE水平的灵敏度更高.

科学领域:

生物化学生物化学
临床化学临床化学
神经学神经学

背景情况:

大脑脊髓液 (CSF) 血管酶转化酶 (ACE) 活性对于诊断神经沙尔科毒症至关重要.
在CSF中评估ACE确定方法对于准确的临床评估至关重要.

研究的目的:

为了比较放射测量和光谱测量试验的性能特征,用于在CSF中ACE的确定.
为了评估这些动力测试与酶链接免疫吸收测试 (ELISA) 进行ACE测量.

主要方法:

通过放射测量用[glycine-1-14C] benzoyl-L-histidyl-L-leucine和光谱测量用furylacryloyl-phenylalanyl-L-glycyl-L-glycine (FAPGG) 基板进行了57个CSF样本的研究.
将动力测试与人类ACE特定ELISA进行比较.
分析了测试精度,检测和量化极限以及量化范围.

主要成果:

精度差在ELISA的5-8%,放射测量的14-17%和光谱测量的6-19%之间.
放射测量证明了检测的最低极限 (0.04 U/L),超过光谱相光测量 (1.0 U/L) 和ELISA (0.156 μg/L).
测试之间观察到良好的相关性,尽管动力测试测量活性,而ELISA测量ACE分子.

结论:

放射测量比光谱测量更敏感,用于CSF ACE的确定,光谱测量的检测极限往往超过病理水平.
艾丽莎测试为放射测量提供了一个潜在的替代方案,等待进一步评估,规范化和临床实用性评估.
迫切需要对生物流体,特别是脑脊髓液中ACE的确定进行标准化.

关键词:

血管氨酸转化酶 - 血管氨酸转化酶大脑脊髓液中的脑脊液.与酶相关的免疫吸收剂测定 (ELISA)这是一种神经沙尔科毒症.射线测量仪的射线测量方法频谱光度测量是一种光谱光度测量.

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