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基于液体的细胞学固定溶液对免疫细胞化学的影响:细胞学样本中抗原检索的有效性

Nanako Sakabe¹, Sayumi Maruyama¹, Chihiro Ito¹

¹Pathophysiology Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Diagnostic cytopathology

|June 17, 2023

概括

基于液体细胞学 (LBC) 样本的免疫细胞化学 (ICC) 显示了抗原检索 (AR) 的改善结果. 然而,ICC的最佳染色取决于所使用的特定抗原,细胞类型和LBC固定溶液.

科学领域:

细胞病理学细胞病理学
免疫组织化学免疫组织化学
诊断病理学的诊断病理学

背景情况:

免疫细胞化学 (ICC) 提高了细胞学中的诊断准确性.
基于液体的细胞学 (LBC) 是一种常见的样本制备方法.
适当的固定对于LBC标本上成功的ICC至关重要.

研究的目的:

调查LBC固定解决方案对ICC的影响.
评估针对ICC的LBC标本中抗原检索 (AR) 的疗效.

主要方法:

使用了五种类型的LBC固定样本 (SurePathTM) 与细胞系.
使用13个抗体进行了ICC.
通过定量染色样本中的阳性细胞来分析结果.

主要成果:

热诱导的AR (HIAR) 显著提高了对核抗原的ICC反应性.
反应性根据LBC固定溶液 (CytoRichTM Blue,CytoRichTM Red,TACASTM Ruby) 和特定抗原 (例如,Ki-67,雌激素受体,p63,细胞克拉5/6) 的不同而有所不同.
细胞质和细胞膜抗原在没有HIAR和某些LBC固定剂的情况下显示出减少的反应性.

结论:

对LBC标本的ICC是一个有价值的诊断工具.
染色条件,包括选择LBC固定溶液和需要AR,必须针对每个抗原进行优化.
抗原,细胞类型和固定剂之间的相互作用会影响ICC免疫反活性.

关键词:

细胞学样本样本热诱导的抗原检索免疫细胞化学免疫细胞化学基于液体的细胞学.

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