分散式液体 - 液体微提取与酶相关免疫吸收试验相结合,用于谷物样本中pyrifos的分析
Zhenjia Chen1, Luyao Zhao2, Zhuoting Zhang1
1College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Talanta
|June 17, 2023
概括
一种新方法有效地检测谷物中的pyrifos (CPF),使用微提取和敏感的免疫测试. 这种方法可以准确快速地分析食品样本中的农药残留物.
科学领域:
- 农业化学 农业化学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- pyrifos (CPF) 是一种广泛使用的有机酸盐杀虫剂.
- 准确检测食品中的CPF残留物对于消费者安全和监管合规至关重要.
- 现有的CPF分析方法可能耗时,需要复杂的样本准备.
研究的目的:
- 开发和验证一种新,灵敏和快速的方法,用于谷物矩阵中的pyrifos分析.
- 将分散液-液微提取与与酶相关的免疫吸收剂测定相结合,以提高CPF检测.
- 在线性,检测极限,回收和精度方面评估方法的性能.
主要方法:
- 分散式液体-液体微提取,使用深溶性溶剂和脂肪酸进行CPF提取和缩.
- 与酶相关的免疫吸收试验 (ELISA) 使用金纳米粒子和磁珠用于信号放大和快速检测.
- 使用标准谷物样本 (大米,小麦,玉米,小米) 验证该方法.
主要成果:
- 该方法实现了pyrifos的0.002-1μg kg-1的广泛线性范围.
- 获得了0.0006μg kg-1的低检测极限.
- 证明了高提取回收率 (86.7-99.9%) 和良好的精度 (RSD < 7.0%).
结论:
- 拟议的方法提供了一种灵敏,高效和快速的方法,用于谷物中pyrifos残留物分析.
- 微提取和免疫试验的结合为常规食品安全监测提供了一个有希望的替代方案.
- 该方法显示了在检测CPF和其他农药残留物在各种食品矩阵中的应用潜力.
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