七素调节边界细胞表面的几何形状,形状和运动性,在Drosophila的Rho下游调节
Allison M Gabbert1, Joseph P Campanale1, James A Mondo1
1Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Developmental cell
|June 17, 2023
概括
七通过调整表面特性来调节细胞群的形状和运动. 罗控制隔膜局部化,影响Drosophila的细胞脱离和运动.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 生物物理学的生物物理.
背景情况:
- 七是已知的蛋白质聚合物,可以在体外结合和变形膜.
- 它们的体内功能和调节正在积极研究中.
- 七因涉及到不同的细胞行为.
研究的目的:
- 为了研究隔膜在边界细胞集群脱离和Drosophila的运动性中的作用.
- 了解 septin 特性如何与它们的 in vivo 功能有关.
- 阐明控制隔膜局部化和功能的调节机制.
主要方法:
- 在Drosophila边界细胞群中对septins和myosin的动态成像.
- 对septin和myosin的局部化和共享表型的分析.
- 研究Rho在调节隔膜和肌肉蛋白中的作用.
- 数学建模以分析隔膜表达水平对集群形态学的影响.
主要成果:
- 七和肌在边界细胞群外围局部化,但不会直接相互影响.
- 罗独立调节肌肉蛋白活性和隔膜局部化.
- 活跃的Rho将隔膜招募到膜上,而不活跃的Rho将它们隔离到细胞质中.
- 七号表达水平在不同尺度上差异调节集群表面纹理和形状.
结论:
- 七调整了表面的变形性,而髓控制了收缩性,共同控制了集群形状和运动.
- 罗作为一个关键的调节器,控制隔膜招募到膜.
- 这项研究揭示了septin水平如何通过表面性质调节来调节细胞群动力学.
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