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pH 对 Anopheles gambiae 臭剂结合蛋白 OBP1 和 OBP4 间的醇依赖异构体相互作用的影响

Bhavika Mam¹, Katerina E Tsitsanou², Panagiota G V Liggri³

¹National Centre for Biological Sciences, Bangalore, Karnataka, India; The University of Trans-Disciplinary Health Sciences and Technology (TDU), Bangalore, Karnataka, India.

International journal of biological macromolecules

|June 17, 2023

概括

昆虫嗅觉蛋白 (OBPs) 随着pH值的变化而改变形状,影响着气味结合. 这项研究揭示了pH和内醇如何影响OBP1-OBP4异构体的形成,这对于蚊子的嗅觉至关重要.

科学领域:

生物化学生物化学
结构生物学结构生物学
昆虫生理学昆虫生理学

背景情况:

昆虫的气味结合蛋白 (OBPs) 对于嗅觉至关重要,调解气味识别.
OBPs表现出pH取决于形状的变化,并且可以形成具有改变结合性质的异构体.
安诺菲尔斯·冈比亚 (Anopheles gambiae) OBP1和OBP4可能会形成异构体,以进行特定的感知.

研究的目的:

阐明OBP4-英多尔相互作用的结构基础.
研究OBP1和OBP4之间的pH依赖的异构化机制.
了解pH值和内醇结合如何影响OBP稳定性和复合物形成.

主要方法:

在不同的pH值下对OBP4进行X射线晶体学.
光竞争试验测试道结合.
分子动力学 (MD) 模拟和差分扫描热量计 (DSC) 用于稳定性研究.
对OBP1-OBP4异构体进行计算建模.

主要成果:

OBP4经历了pH取决于形状的变化,特别是在N端和α4-循环-α5区域.
印度尔与OBP4的结合较弱,在酸性pH下进一步受损.
pH显著影响OBP4的稳定性,比内更重要.
更高的pH值稳定了OBP4,可能有助于印醇结合和OBP1相互作用.
酸性pH会破坏OBP1-OBP4异构体接口的稳定,并减少相关的运动.

结论:

为OBP1-OBP4异构体的形成和解离提出了一种pH和醇驱动的机制.
在中性/性条件下OBP4的pH诱导稳定是印结合和异构化的关键.
在酸性pH值下异聚合物解离促进了印醇的释放,调节了嗅觉信号.

关键词:

这是一种异构聚合物 (heterodimer).印度尔 (Indole) 是一种昆虫的嗅觉是昆虫的嗅觉.接口的能量学.分子动力学分子动力学蚊子蚊子蚊子在OBP系统上设置OBP系统.气味剂结合蛋白质是气味剂结合蛋白质的一种.结构结构是一个结构.在pH的依赖性.

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