二二三乙通过改变氧化氨的古生物和细菌群体来抑制土壤氨氧化
Jing Chen1, Shuo Zhao2, Yating Gan1
1College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China.
Environmental pollution (Barking, Essex : 1987)
|June 17, 2023
概括
DDT污染最初会抑制土壤氨氧化,但随着时间的推移会恢复. 持久有机污染物影响氨氧化古生物 (AOA) 的多样性和社区结构,影响关键的土壤微生物过程.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 生物地质化学生物地质化学
背景情况:
- 二二三乙 (DDT) 是一种持久性有机污染物,已知对微生物群落产生影响.
- 对于DDT对土壤氨氧化古生物 (AOA) 和氨氧化细菌 (AOB) 的影响,这些细菌是土壤化中的关键参与者,它们在很大程度上尚未被探索.
- 了解这些影响对于评估DDT污染对土壤健康和营养循环的更广泛环境影响至关重要.
研究的目的:
- 调查DDT污染对土壤氨氧化率的影响.
- 分析DDT对土壤AOA和AOB的丰富性,多样性和社区组成的影响.
- 阐明DDT,其代谢物及其土壤转化过程之间的关系.
主要方法:
- 一个为期30天的微观宇宙实验旨在模拟土壤中的DDT污染.
- 在实验期间测量了土壤氨氧化率.
- 使用定量PCR (qPCR) 来量化阿莫A基因在AOA和AOB社区的丰度.
- 分析了AOA和AOB的社区组成和多样性.
- 在微生物数据,物种 (NH4+-N,NO3--N) 和DDT/代谢物度之间进行了统计相关性.
主要成果:
- DDT最初抑制了土壤氨氧化 (0-6天),但在16天后观察到恢复.
- 暴露于DDT导致AOA中的amoA基因拷贝数量减少,并在AOB中暂时减少.
- DDT显著改变了AOA的多样性和社区结构,在Nitrososphaera sp.中观察到特定的相关性. JG1和未培养的氨氧化克雷纳古生物.
- 虽然Nitrosospira sp. 的影响较小,但AOB社区的组成受影响较小. III7显示与DDT及其代谢物有负相关性.
结论:
- DDT及其代谢物通过影响AOA和AOB社区对土壤氨氧化过程产生重大影响.
- 该研究强调了AOA和AOB对DDT污染的不同敏感性,AOA在社区结构中显示出更大的转变.
- 这些发现强调了持久性有机污染物有可能破坏关键的土壤循环功能.
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