新的光谱光度和基于智能手机的色度测量方法用于药物配方中的阿提诺洛尔的测定
Rebwar Omar Hassan1, Hazha Omar Othman2, Diyar Salahuddin Ali3
1Department of Chemistry, College of Science, Salahaddin University, Erbil-Kurdistan Region, Iraq.
概括
新的光谱光度和基于智能手机的色度测量方法准确地估计了药品中的阿诺醇 (ATE). 这些方法利用了脱化反应,为药物分析提供了可靠的替代方案.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
背景情况:
- 阿诺醇 (ATE) 是一种广泛用于治疗心血管疾病的β-阻断剂.
- 药品配方中ATE的准确量化对于质量控制和患者安全至关重要.
- 现有的分析方法可能需要专门的设备或复杂的程序.
研究的目的:
- 开发和验证新的光谱光度和基于智能手机的色度测量 (SBC) 方法来估计阿提诺洛尔 (ATE).
- 将开发方法的性能与标准HPLC方法进行比较.
- 评估这些方法在药品片中分析ATE的适用性.
主要方法:
- 开发了一种基于抑制495 nm的染料形成反应的光谱光度测量方法.
- 使用智能手机摄像头和RGB应用程序开发SBC方法来量化颜色强度.
- 使用中央复合设计 (CCD) 和响应表面方法来优化反应剂度.
- 对线性,干扰,检测极限 (LOD) 和量化极限 (LOQ) 的方法的验证.
主要成果:
- 这两种方法在8.0至60.0微克毫升-1.1的度范围内都表现出良好的线性.
- 光谱光度法显示R2 = 0.9993,LOD = 1.28μg mL-1和LOQ = 4.28μg mL-1.1. 这两种情况均为相同.
- 在SBC方法中显示R2 = 0.9965,LOD = 2.13μg mL-1和LOQ = 7.09μg mL-1.
- 对于这两种方法都没有观察到任何显著干扰.
- 统计比较 (t-试验和F-试验) 证实了针对HPLC开发的方法的有效性.
结论:
- 新型,敏感和验证的光谱和SBC方法已成功开发用于atenolol量化.
- SBC 方法为制药分析提供了一个具有成本效益和可访问性的替代方案.
- 这些方法适用于药物配方中阿提诺洛尔的常规分析.
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