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使用权重基因联合表达网络分析 (WGCNA) 识别导致皮肤雷什曼病的皮肤病变的分子机制

Kavoos Momeni¹, Saeid Ghorbian², Ehsan Ahmadpour³

¹Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran.

Scientific reports

|June 17, 2023

概括

皮肤莱什曼病会导致不愈合的皮肤伤口和痕. 生物信息学确定了破坏组织修复的关键基因,为这种被忽视的热带疾病提供了潜在的新治疗点.

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
皮肤病学皮肤病学

背景情况:

莱什曼病是一种由原生动物寄生虫引起的传染病.
皮肤莱什曼病是最常见的形式,导致不愈合的皮肤伤口和永久性痕.
皮肤莱什曼病的标准治疗具有很高的失败率,需要新的治疗策略.

研究的目的:

通过联合生物信息学分析,在健康皮肤中识别差异表达基因 (DEGs),而不是使用Leishmania感染的皮肤伤口.
分析WGCNA模块和基因本体学功能,以了解伤口愈合障碍背后的分子机制.
确定关键的基因和途径参与皮肤莱什曼病引起的皮肤损伤的发病.

主要方法:

联合生物信息学分析健康的皮肤和Leishmania皮肤伤口活检.
权重基因共同表达网络分析 (WGCNA) 以确定与伤口大小相关的基因模块.
功能性丰富分析使用基因本体学和Cytoscape进行途径识别.

主要成果:

在Leishmania伤口周围的皮肤中确定了近16,600个DEG.
一个特定的456个基因的WGCNA模块显示了与伤口大小最强的相关性.
该模块含有参与产生损害组织的细胞因子和破坏原/细胞外基因生产的基因,阻碍伤口愈合. 中枢基因包括OAS1,SERPINH1和FBLN1.1.

结论:

已识别的基因模块和枢纽基因为皮肤莱什曼病中伤口愈合受损的分子机制提供了洞察力.
针对这些特定的基因或途径可能会导致针对不愈合的皮肤莱什曼病伤口的新型治疗干预措施.
这项研究为开发治疗方法提供了基础,以减轻皮肤莱什曼病的有害影响并改善患者的治疗结果.

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