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在储存期间,在白色按 (Agaricus bisporus) 中预测细菌群体动态和代谢功能

Fanyun Hou¹, Fangxuan Yi¹, Lisha Song¹

¹School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255049 Shandong, PR China.

Food research international (Ottawa, Ont.)

|June 18, 2023

概括

白色按 (Agaricus bisporus) 中的细菌破坏是由 Pseudomonas 驱动的,特别是 Pseudomonas tolaasii. 这项研究确定了关键的细菌和代谢途径,为延长的保质期提供了见解.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
生物信息学是一种生物信息学.

背景情况:

白色 (Agaricus bisporus) 营养丰富,但在储存期间容易受到微生物破坏.
损坏减少了保质期和经济价值,需要了解所涉及的微生物动态.

研究的目的:

研究A. bisporus储存期间细菌群落多样性和代谢功能的变化.
为了识别导致腐烂和黑斑形成的致病细菌.
为制定延长保质期的策略提供基础.

主要方法:

在不同的存储时间进行A. bisporus的高通量测序 (Illumina Novaseq 6000).
使用QIIME2和PICRUSt2进行生物信息分析,用于细菌多样性和代谢功能的预测.
从腐烂的样本中分离,识别和检测细菌的病原性.

主要成果:

在储存期间,细菌物种的丰富性下降,而Pseudomonas的丰富性显著增加,成为主要的腐烂细菌.
预测了代谢途径,特别是一般代谢,Pseudomonas与13个功能途径正相关.
鉴定出 Pseudomonas tolaasii 是一个关键的病原体,导致了 A. bisporus 的显著破坏.

结论:

伪菌,特别是P. tolaasii,在储存的白色按菌的腐烂中起着至关重要的作用.
了解这些微生物的动态和代谢功能可以指导开发有效的保存策略.
这项研究支持制造抗菌溶液,以提高的存储寿命.

关键词:

亚加里克斯·比斯波鲁斯 (Agaricus bisporus) 是一种植物.社区结构社区结构代谢功能的预测和预测伪omonas tolaasii 这种类型的

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