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在土豆中,CRISPR/Cas9介导的多导向RNA向性突变发生

Laura Abeuova^1,2, Balnur Kali¹, Dilnur Tussipkan¹

¹National Center for Biotechnology (NCB), Astana, 010000, Kazakhstan.

Transgenic research

|June 18, 2023

概括

通过CRISPR/Cas9基因编辑,在土豆中有效地产生了多种突变.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

CRISPR/Cas9是植物的主要基因组编辑工具.
马是重要的工业作物,需要先进的基因改造技术.

研究的目的:

使用CRISPR/Cas9.9开发三或四基基突变的土豆系.
为了在土豆中有效地敲除颗粒结合粉合成酶 (gbss) 基因.

主要方法:

设计和建造了针对 gbss exon I 的三个区域的 CRISPR/Cas9 表达载体.
采用Agrobacterium介导的转化方法,将CRISPR/Cas9系统引入土豆中.
使用CAPS,桑格测序和染来分析突变.

主要成果:

在gbss基因中产生了具有多个核酸替代和indels的土豆线.
实现了框架转移突变,导致过早停止密码子和gbss基因淘汰.
确认成功的基因淘汰,导致无粉素的表型.

结论:

在马基因组编辑方面,CRISPR/Cas9介导的多导向RNA突变发生非常有效.
这种方法可以有效地产生gbss-knockout的土豆植物.
由此产生的无粉土豆具有重要的工业应用潜力.

关键词:

氨糖糖是什么氨糖是什么在CAPS分析中,分析了CAPS.这就是CRISPR/Cas9的作用.这就是为什么GBSS是GBSS.S. 结核体 L 结核体

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