乳球球菌的高生物多样性是否允许预测它们不同的UV-C易感性?
Christina Schubert1, Natalia Biere2, Erik Brinks2
1University of Hohenheim, Institute of Food Science and Biotechnology, Department of Soft Matter Science and Dairy Technology, Garbenstraße 21, D-70599 Stuttgart, Germany.
International journal of food microbiology
|June 18, 2023
概括
菌体通过破坏初始培养物来威胁发酵. 将膜过和UV-C辐射相结合有效地消除了乳清中的这些威胁,确保了更安全的使用和过程连续性.
科学领域:
- 食品微生物学 食品微生物学
- 生物技术是生物技术.
- 工艺工程是过程工程.
背景情况:
- 菌体对发酵过程构成重大风险,特别是在奶酪生产中,通过溶解必要的细菌初始培养物.
- 乳清是奶酪生产的副产品,可能受到细菌菌体的严重污染,对其利用构成质量和加工风险.
研究的目的:
- 为了评估一个合并膜过和UV-C辐射以消除乳清中的菌体的直角过程.
- 通过评估各种乳球球菌的紫外线抗性来确定适合细菌失活的过程参数.
主要方法:
- 查11种乳球菌菌对乳清中的UV-C耐药性的查,考虑了形态学和基因组大小等属性.
- 膜过的应用 (达到4个逻辑单位的减少),然后进行UV-C辐射 (目标是5个逻辑单位的减少).
- 在菌体P008上进行突变实验,以评估抗UV-C的潜在发展.
主要成果:
- 乳球球菌P369被确定为最耐UV-C,适合作为生物标志物.
- 在初始膜过后,UV-C剂量为5J/cm2实现了细菌菌体的5日志单位减少.
- 在UV-C敏感性和菌体形态或基因组大小之间没有明确的相关性.
- 突变实验没有诱导细菌P008.8显著的UV-C耐药性.
结论:
- 膜过和UV-C辐射的联合过程是有效的产生无菌体乳清.
- 该过程可能会随着时间的推移保持其有效性,因为没有观察到诱导抵抗.
- 可能需要进一步的研究来确定影响UV-C敏感性的所有参数.
更多相关视频
09:59Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance
Published on: July 21, 2023
1.3K
03:33Author Spotlight: An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection
Published on: June 30, 2023
2.2K
相关概念视频
01:30Lytic Cycle of Bacteriophages
71.1K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
71.1K
01:16Viral Replication: Lysogenic Cycle
87
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
87
00:43Lysogenic Cycle of Bacteriophages
62.6K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.6K
01:26DNA Bacteriophages
62
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
62