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在胃癌中,PRSS2通过MMP-9调节EMT和转移
Fei Wang1, Jianfeng Yi2, Yu Chen2
1Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China; Department of General Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
Acta histochemica
|June 18, 2023
概括
血清蛋白酶2 (PRSS2) 通过通过矩阵金属蛋白酶-9 (MMP-9) 诱导上皮细胞-介质细胞过渡 (EMT) 来促进胃癌转移. PRSS2可以作为胃癌的早期诊断标记物和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 血清蛋白酶2 (PRSS2) 在胃癌中被上调.
- PRSS2与不良预后相关,并促进胃癌细胞迁移和入侵.
- 在胃癌转移中PRSS2的确切机制尚不清楚.
研究的目的:
- 研究PRSS2在胃癌转移中的作用和机制.
- 分析血清PRSS2水平,临床病理特征和胃癌患者的MMP-9表达之间的相关性.
- 评估PRSS2作为潜在的早期诊断标记物和治疗点.
主要方法:
- 在胃癌患者和健康对照中使用ELISA测量血清PRSS2水平.
- 进行了血清PRSS2,临床病理特征和MMP-9水平之间的相关性分析.
- 具有PRSS2沉默的胃癌细胞被MMP-9过度表达载体感染,以评估迁移,入侵和EMT.
主要成果:
- 在胃癌患者中观察到血清PRSS2水平升高,与淋巴转移和TNM阶段有关.
- 血清PRSS2水平与血清MMP-9水平呈正相关性.
- PRSS2沉默抑制了EMT,而PRSS2倒置部分逆转了转移和EMT,由MMP-9过度表达引起.
结论:
- 通过MMP-9介导的EMT诱导,PRSS2促进胃癌细胞的迁移和入侵.
- PRSS2被认为是胃癌的潜在早期诊断生物标志物.
- 在胃癌治疗中,PRSS2是一个有前途的治疗标.
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