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一种新的C7蛋白的功能性鉴定番茄黄色叶子卷曲病毒的C7蛋白

He Liu¹, Zhaoyang Chang², Siwen Zhao²

¹College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, 071000, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

|June 18, 2023

概括

一种新发现的番茄黄色叶子卷曲病毒 (TYLCV) 蛋白质C7作为一种致病性因素. 这种病毒蛋白延迟感染,减少症状,并抑制RNA沉默,在TYLCV疾病中发挥关键作用.

科学领域:

植物病毒学植物病毒学
分子植物病理学分子植物病理学
病毒与宿主之间的相互作用

背景情况:

番茄黄色叶子卷曲病毒 (TYLCV) 是一种主要的双子病毒,影响着世界各地的番茄作物.
传统上,TYLCV编码六种蛋白质,但最近的发现表明,还有其他小型病毒蛋白质.
这些新型蛋白质可能具有独特的亚细胞定位和毒性功能.

研究的目的:

为了识别和表征一种由TYLCV.编码的新型蛋白质.
研究这种新蛋白在病毒感染和病原性中的作用.
为了确定蛋白质的细胞下定位,相互作用,并作为RNA沉默抑制剂的功能.

主要方法:

质谱法用于识别新型C7蛋白.
亚细胞局部化研究是在病毒存在或不存在的情况下进行的.
研究了与TYLCV蛋白C2和V2的蛋白质-蛋白质相互作用.
使用PVX载体采用C7起始编码子和子宫外表达的突变发生.
进行了GFP诱导的RNA沉默试验.

主要成果:

一种新的TYLCV蛋白,C7被确定,局部化到细胞核和细胞质.
C7与TYLCV蛋白C2 (核) 和V2 (细胞质) 相互作用.
突变C7起始编码子延迟了感染,减少了病毒症状,并减少了病毒DNA/蛋白质积累.
宫外C7表达加剧了马赛克症状,增加了PVX外衣蛋白积累.
发现C7是RNA沉默的中度抑制剂.

结论:

新型C7蛋白是TYLCV.的重要致病因子.
C7作为一种弱弱的RNA沉默抑制剂起作用.
这种蛋白质在TYLCV感染周期和疾病发展中发挥着关键作用.

关键词:

C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 C7 新型致病因子新型致病因子制止RNA沉默抑制器抑制器番茄黄色叶子卷曲病毒病毒

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