在同人类状体中识别了新的天体病毒
Georgia Diakoudi1, Alessio Buonavoglia1, Francesco Pellegrini1
1Department of Veterinary Medicine, University of Bari, Valenzano, Italy.
Research in veterinary science
|June 18, 2023
概括
在意大利的和身上发现了天体病毒,在一只动物的大脑中发现了病毒RNA. 这一发现突显了天体病毒的遗传多样性,并表明了爬行动物物种之间的潜在传播.
科学领域:
- 兽医病毒学 兽医病毒学
- 爬行动物传染病 爬行动物传染病
- 分子流行病学分子流行病学
背景情况:
- 天体病毒是众所周知的各种动物和人类的病原体,经常导致胃肠道疾病.
- 在各种宿主中记录了星际病毒感染的肠外表现.
- 类似人类的爬行动物,如和,可以携带新型病毒原体.
研究的目的:
- 为了调查意大利南部同类状爬行动物中天体病毒的存在和遗传多样性.
- 在这些爬行动物种群中检测到的新型天体病毒菌株的特征.
- 探索潜在的肠外天文病毒感染和物种间传播事件.
主要方法:
- 在城市和城市周边地区从100只状爬行动物 (Podarcis siculus和Tarentola mauritanica) 收集便样本.
- 使用广泛反应的 (泛天体病毒) 逆转录聚合酶链反应 (RT-PCR) 检测天体病毒RNA,以向RNA依赖的RNA聚合酶 (RdRp) 基因.
- 从基因组的3'端对6个阳性菌株进行3kb片段的测序,包括完整的囊编码ORF2序列.
主要成果:
- 在11%的抽样爬行动物中检测到天体病毒RNA.
- 测序显示ORF2基因与已知的爬行动物天体病毒相比具有较低的核酸标识 (<43.7%),表明了显著的遗传多样性.
- 在一个阳性动物的大脑组织中发现了病毒RNA,这表明潜在的肠外疾病.
- 对部分RdRp基因的分析显示了特定物种的模式,并表明了和之间可能发生的物种间传播事件.
结论:
- 这项研究报告了首次在意大利的同类状爬行动物中检测到天体病毒.
- 这些发现强调了Astroviridae家族内相当大的遗传多样性,特别是在爬行动物菌株中.
- 在大脑组织中检测到的天体病毒和物种间传播的证据要求进一步调查这些病毒在爬行动物的致病性和流行病学.
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