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在乳受体缺乏的db/db糖尿病病小鼠中的代谢重新连接和表观遗传重编程

Md Shahid Sarwar¹, David Cheng², Rebecca Mary Peter²

¹Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.

European journal of pharmacology

|June 18, 2023

概括

糖尿病病 (DN) 的高葡萄糖会改变代谢和DNA甲基化. 这项研究揭示了这些变化,包括S-adenosylmethionine (SAM) 途径,如何影响db/db小鼠的基因表达和DN进展.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
代谢学代谢学代谢学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

糖尿病病 (DN) 是末期病的主要原因.
线粒体代谢和表观遗传学都与DN发育和进展有关.

研究的目的:

研究高葡萄糖 (HG) 对db/db小鼠脏细胞代谢,DNA甲基化和转录组的影响.
用多学科的方法进行全面的分析.

主要方法:

使用液态染色体质谱法 (LC-MS) 的代谢学.
通过下一代测序 (RNA-seq) 分析的表观基因CpG甲基化和转录基因表达.

主要成果:

HG调节了关键代谢物 (例如,S-adenosylmethionine) 和代谢途径.
TGFβ1和促炎途径参与了早期的DN.
鉴定出差异甲基化的区域和基因 (例如,Cyp2d22,Slc1a4,Ddah1) 与DNA甲基化和基因表达的持续变化.

结论:

素受体缺乏和HG诱导脏中的代谢重新连接.
S-adenosylmethionine (SAM) 可能驱动DNA甲基化和转录组变化,导致DN进展.

关键词:

通过DNA甲基化.糖尿病脏病糖尿病脏病表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.基因表达基因表达基因表达代谢过程中的代谢.在TCA循环中,TCA循环是

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