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在蓝藻细菌中二次代谢的代谢流体表型化

Piyoosh K Babele¹, Amit Srivastava², Jamey D Young³

¹College of Agriculture, Rani Lakshmi Bai Central Agricultural University Jhansi, 284003, Uttar Pradesh, India.

Trends in microbiology

|June 18, 2023

概括

菌可以被设计为更好的生物生产. 系统代谢工程,使用代谢流量分析,可以优化这些微生物的有价值化合物的产量.

科学领域:

微生物学微生物学
生物技术是生物技术.
代谢工程是代谢工程.

背景情况:

菌是一种光合作用微生物,具有产生有价值的二次代谢产物的潜力.
目前的挑战包括低产品产量,标位和利率,阻碍它们作为生物生产平台的使用.
需要取得进展,以使蓝藻细菌成为首选的微生物工厂.

研究的目的:

审查代谢流量分析 (MFA) 和系统代谢工程 (SME) 的潜力,以优化蓝藻细菌的二次代谢物产量.
讨论与在蓝菌中实施MFA和SME相关的技术挑战.
为突出提高蓝藻细菌生物生产的战略.

主要方法:

代谢流量分析 (MFA) 用于定量评估细胞内碳流.
系统代谢工程 (SME) 将MFA与其他omics数据集成.
对蓝藻细菌的代谢和调节途径的分析.

主要成果:

MFA阐明了通过转录,转化和质机制调节代谢途径.
中小企业为微生物生产中的合理菌株开发提供了框架.
该审查强调了MFA和SME在蓝菌应用中的巨大潜力.

结论:

MFA和SME是了解和改进蓝藻细菌代谢网络的强大工具.
克服MFA和SME的技术挑战对于释放蓝藻细菌的生物生产潜力至关重要.
进一步的研究是必不可少的,以建立蓝色细菌作为各种化合物的领先的生物生产平台.

关键词:

基因组规模的代谢模型代谢流量分析的代谢流量分析.二次新陈代谢二次新陈代谢二次代谢产物二次代谢产物系统代谢工程代谢工程

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