近期去世的个体中COVID-19病毒的量化和持久性在埋葬前和之后的埋葬
Lorenzo Gitto1, Frank A Middleton2,3,4, Erin S Reynolds5,6,7,8
1Department of Pathology, Cook County Medical Examiner's Office, Chicago, Illinois, USA.
Anatomical sciences education
|June 19, 2023
概括
化显著减少了尸体中可行的COVID-19,但仍需要采取预防措施. 肺组织是检测死者的病毒的最佳指标.
科学领域:
- 医疗教育 医学教育
- 传染性疾病 传染性疾病
- 病毒学 病毒学
背景情况:
- 在全球范围内,COVID-19大流行扰乱了医学教育.
- 对于学生和工作人员来说,从COVID-19阳性尸体感染的风险尚不清楚.
- 医学院拒绝COVID-19阳性尸体已经阻碍了教育.
研究的目的:
- 为了比较Covid-19病毒基因组在已故捐赠者的组织中的丰富性,在化前和化后.
- 评估这些组织中传染性病毒存在的可能性.
- 确定在教育和研究环境中使用防腐化COVID-19阳性尸体的安全性.
主要方法:
- 组织样本 (肺部,肝脏,脏,大脑) 从四名COVID-19阳性捐赠者中收集,分别在化前和后进行.
- 通过对A549-hACE2细胞注射人体组织同质物来测试感染性病毒的存在.
- 实时定量逆转录聚合酶连锁反应 (RT-qPCR) 定量病毒RNA.
主要成果:
- 病毒基因组在死后的几天内也可以在组织中检测到.
- 在所有测试的组织中,大量减少可行的COVID-19基因组,往往达到无法检测的水平.
- 传染性病毒和可检测的RNA仍然存在于一些包装前和后的组织中,肺组织是最可靠的指标.
结论:
- 包的COVID-19阳性尸体可以安全地用于粗体解剖实验室和研究,并采取适当的预防措施.
- 肺组织是检测可行的COVID-19存在的最佳样本.
- 肺组织的负结果表明其他组织的积极结果的可能性非常低.
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