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验证一种临床上适用的设备,用于快速准确地量化血液体积
Andreas Breenfeldt Andersen1, Thomas C Bonne2, Joar Hansen3
1Department of Public Health, Research Unit for Exercise Biology, Aarhus University, Aarhus, Denmark.
Journal of clinical laboratory analysis
|June 19, 2023
概括
一个新的半自动设备使用一氧化碳再呼吸精确测量血液体积 (BV),与双同位素方法相对应. 这种更快,无痕迹剂的方法可以在临床环境中频繁监测BV.
科学领域:
- 身体生理学 身体生理学
- 医疗器械 医疗器械
- 诊断方法 诊断方法
背景情况:
- 传统的双同位素法用于血液体积 (BV) 测定,因同位素半衰期长而受到限制.
- 一氧化碳 (CO) 再呼吸程序已经在实验室中使用了100年,使频繁的BV测量成为可能.
研究的目的:
- 评估半自动化CO再呼吸装置用于血液体积 (BV) 测量的可靠性和准确性.
- 为了比较二氧化碳再呼吸装置与已建立的双同位素方法.
- 评估该设备检测血液体积中已知小变化的能力.
主要方法:
- 研究了一种用于血液体积 (BV) 测定的半自动化CO再呼吸装置.
- 与A研究中的双同位素技术对比的设备测量 (2分钟和10分钟协议)
- 在B研究中,通过测量其检测BV减少2%的能力来评估设备的准确性.
主要成果:
- 在CO再呼吸方案和双同位素方法 (p < 0.001) 之间发现了高相关性 (r2 = 0.89-0.98).
- 双同位素方法产生的BV值较低 (425-491毫升,p<0.001),相比于CO再呼吸.
- 该装置准确量化了150 ± 45毫升 (p < 0.001) 的BV减少,相当于2%的变化.
结论:
- 半自动化CO再呼吸装置准确地测量血液体积 (BV) 的小变化 (2%).
- 该方法与双同位素技术具有很高的相关性.
- 由于速度 (~15分钟),无放射性痕迹物以及适合重复测量而具有临床意义.
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