生物分析物的温度梯度聚焦在处理非牛顿电解质的微流体通道中,考虑到温度依赖的泽塔潜力
Amitava Dutta1, Apurba Kumar Santra2, Ranjan Ganguly2
1Department of Mechanical Engineering, Aliah University, Kolkata, India.
Electrophoresis
|June 19, 2023
概括
温度梯度聚焦 (TGF) 使用电场和流体流来将分析物集中在微通道中. 非牛顿流体粘度显著影响分析剂度,而牛顿行为产生了最高的增强.
科学领域:
- 微流体学 微流体学
- 分析化学 分析化学
- 生物物理学的生物物理.
背景情况:
- 温度梯度聚焦 (TGF) 是一种微流体技术,用于分析物度.
- 它依赖于平衡分析物的电泳流动性和背景电解质 (BGE) 辅助流.
- 了解BGE的风湿学对于优化TGF至关重要.
研究的目的:
- 通过TGF通过数值分析非牛顿BGE粘度对分析剂度的影响.
- 为了研究温度依赖的墙壁泽塔电位和BGE流动行为指数 (n) 的影响.
主要方法:
- 结合电场和传输方程的有限元数值分析.
- 在微通道中模拟朱尔加热.
- 模拟非牛顿BGE形学和温度依赖的泽塔潜力.
主要成果:
- 分析物的度随着温度依赖的泽塔电位的增加而下降.
- 随着牛顿式BGE形学 (n=1) 发生最大度增强.
- 伪塑性 (n<1) 和扩张性 (n>1) BGEs与牛顿式 BGEs相比显示出较低的度增强.
结论:
- 在TGF性能方面,BGE质,特别是其切割依赖的粘度,是关键因素.
- 取决于温度的泽塔潜力会对分析器聚焦产生负面影响.
- 优化BGE质学是最大限度地提高微流体器件中分析物度的关键.
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