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一个人工培养发酵系统用于工业醇生产

Rémi Hocq^1,2, Michael Sauer^1,2

¹CD-Laboratory for Biotechnology of Glycerol, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.

|June 19, 2023

概括

人工微生物共同培养提高了生物燃料的生产. 结合特定的细菌,提高了植物生物质发酵的1-propanol和2-propanol产量,优于单一菌株.

科学领域:

微生物生物技术微生物生物技术
合成生物学合成生物学
生物能源是生物能源.

背景情况:

植物生物质的微生物发酵是可再生生物燃料和生物化学品的关键.
目前的方法通常使用单个微生物菌株,与自然微生物群落不同.
人工共同培养通过结合多种微生物提供了更广泛的代谢能力.

研究的目的:

调查人工共同种植对提高生物燃料生产的潜力.
通过微生物发酵,探索酸盐转化为1-propanol和2-propanol的过程.
优化培条件,以增加溶剂的生产.

主要方法:

使用了包括酸细菌 (PAB) 和溶剂性Clostridia在内的培系统.
测试了连续和同时发酵策略.
多种不同的接种比率的 * Propionibacterium freudenreichii * 和 * Clostridium beijerinckii *.

主要成果:

与P. freudenreichii*和C. beijerinckii*共同培养,与单一培养相比,显著增加了溶剂产量.
达到了21g/l的总溶剂和12g/l的醇.
在共同种植中证明了代谢协同作用,提高了发酵性能.

结论:

人工共同培养可以促进新陈代谢协同作用,以改善生物燃料生产.
这种方法有效地增加了发酵产量,并将碳流向所需产品.
共同培养微生物是将植物生物质提升为有价值的化学物质的有希望的战略.

关键词:

克洛斯特里迪亚姆 (Clostridium) 是一种生物燃料是一种生物燃料.生物技术是生物技术.共同培养是一种共同文化.醇醇是一种酸细菌是一种酸细菌.

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