了解古代人类牙的微生物生物地理,以指导研究设计和解释
Zandra Fagernäs1,2, Domingo C Salazar-García3,4,5, María Haber Uriarte6
1Department of Archaeogenetics, Max Planck Institute for the Science of Human History, 07745 Jena, Germany.
FEMS microbes
|June 19, 2023
概括
古老的口腔微生物组研究得到了验证:牙结石微生物组成的个体间差异超过了个体内差异. 这支持使用单个样本进行古代口腔微生物组研究,同时注意到小的空间模式和死后DNA起源.
科学领域:
- 古生物生物学 古生物生物学
- 口腔生物学 口腔生物学
- 考古遗传学 考古遗传学
背景情况:
- 口腔的异质性会影响牙斑中的微生物群落.
- 考古学研究经常假设一个单一的牙结石样本代表一个人的整个口腔微生物组.
- 这种假设的有效性和潜在的偏见尚未得到充分理解.
研究的目的:
- 在考古牙科微积分中调查微生物组成和空间模式.
- 评估使用单个样本用于古代口腔微生物组研究的可靠性.
- 为了评估植物DNA在牙科微积分中的起源,以进行饮食解释.
主要方法:
- 从四个石灰岩时期 (公元前4500-5000年) 的四个个人的密集采样牙结石的炮弹元基因组测序.
- 分析个体间和个体内微生物组成的差异.
- 在样本中的植物DNA的识别和原产地评估.
主要成果:
- 个人间的微生物差异明显大于个人内差异.
- 在口腔微生物物种分布中观察到较小的空间模式.
- 植物DNA很可能是死后的起源,而不是饮食.
结论:
- 一个单一的牙结石样本通常可以代表一个人的古老的口腔微生物组.
- 意识到微小的空间变化对于研究设计和解释至关重要.
- 环境控制对于从古代DNA准确的饮食重建至关重要.
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