在电子受体和氨限制条件下的混合培养中,Dehalococcoides mccartyi菌株的长期存活率
Nadia Morson1, Olivia Molenda2, Katherine J Picott2
1Department of Cell and Systems Biology, University of Toronto, 25 Harbord St, Toronto, ON M5S 3G5, Canada.
FEMS microbes
|June 19, 2023
概括
在Dehalococcoides mccartyi中,没有诱导化减少酶 (VcrA) 基因组岛的水平基因转移. 相反,由于突变,三乙烯还原酶 (TceA) 调解了化乙烯脱化.
科学领域:
- 环境微生物学环境微生物学
- 生物修复是一种生物修复.
- 基因组学就是基因组学.
背景情况:
- 德哈洛科科伊德 (Dehalococcoides mccartyi) 对于排毒致癌的乙烯基化物 (VC) 是非常重要的.
- 位于基因组岛 (GI) 上的乙烯基化物减少酶 (VcrA) 操作子被认为可以促进水平基因转移 (HGT).
研究的目的:
- 在特定条件下调查vcrA-GI在Dehalococcoides mccartyi水平基因转移 (HGT) 的潜力.
- 探索选择Dehalococcoides mccartyi突变物能够同时进行固和VC呼吸的可能性.
主要方法:
- 两个Dehalococcoides mccartyi丰富培养物被结合在缺乏氨的介质中,并提供VC.
- 化时间超过四年.
- 使用了基因组分析和蛋白质建模.
主要成果:
- 在长时间化后没有观察到vcrA-GI水平基因转移 (HGT) 的证据.
- 乙烯基化物 (VC) 脱是由三乙烯减少酶 (TceA) 介导的,这表明突变改变了其基质特异性.
- 在KB-1文化中发现了两个不同的固Dehalococcoides mccartyi菌株.
结论:
- 这种vcrA-GI的机动性可能比以前预测的要低,或者其机动性仅限于特定的Dehalococcoides亚类.
- 在KB-1培养物中看到的多个Dehalococcoides mccartyi菌株与多种表型的持久性,可能对成功的生物增量策略具有重要意义.
更多相关视频
08:11Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
Published on: June 14, 2024
824
06:45Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains
Published on: January 18, 2014
8.6K
相关概念视频
01:29Diversity of Archaea IV
58
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
58
01:28Microbial Nutrition
115
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
115
01:27Diversity of Archaea III
33
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
33
01:30Diversity of Archaea I
36
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
36
01:28Carbon-dioxide Fixation
43
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
43
01:15Metabolism of Chemolithotrophs
53
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
53