雷多克辛可以调节沙盐应激下Arabidopsis根中的塑性葡萄糖-6-酸盐脱酶活性
Guillaume Née1, Fuzheng Wang1, Gilles Châtel-Innocenti1
1Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2), Gif sur Yvette, France.
Frontiers in plant science
|June 19, 2023
概括
铁素 (TRX) 在盐应激下调节阿拉比多普西斯根中的塑性糖-6-酸脱酶 (G6PDH) 活性. TRXm特别调节G6PDH2和G6PDH3,影响根生长和NADPH的产生.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 植物利用产生NADPH的酶,如葡萄糖-6-酸脱酶 (G6PDH),用于各种代谢过程.
- 塑体G6PDH的活性由氧素 (TRX) 进行氧化还原调节,但异质组织中的调节仍未得到充分研究.
研究的目的:
- 在盐应激下,研究阿拉比多普西斯根中可塑性G6PDH异型的提奥雷多克素 (TRX) 调节.
- 阐明TRXm在调节G6PDH活动中的作用及其对盐应激期间根生长和NADPH产生的影响.
主要方法:
- 在体外测试以确定根G6PDH异型的TRX调节.
- 在盐应激下对G6PD和TRX基因的基因表达分析.
- 在盐应力下对Arabidopsis突变系的表型分析.
- 在现场G6PDH活性测定和反应性氧物种 (ROS) 测定.
主要成果:
- m型TRXs有效调节G6PDH2和G6PDH3,这是Arabidopsis根中的主要塑性异型.
- 盐应激导致特定G6PD和TRX突变系的根生长受损,尽管基因表达发生了轻微的变化.
- 在盐应激下,G6PDH2活性显著增加,TRX m为氧化还原调节提供了体内证据.
结论:
- 塑G6PDH活动的TRX m介导调节对于经历盐应激的Arabidopsis根中的NADPH产生至关重要.
- 这种调节机制在植物适应非生物压力方面发挥着重要作用,影响了根部的发育.
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