转录基因分析揭示了H2S作为天然气传递物的功能,独立于Arabidopsis中的Cys
Huihui Fang1, Zhenyuan Yu1, Kehong Xing1
1Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang, College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang, China.
Frontiers in plant science
|June 19, 2023
概括
硫化 (H2S) 在植物中充当气体传递物,调节基因表达,除了在氨酸合成中的作用之外. 这项研究澄清了H2S信号通路,确定了参与植物发育和应激反应的关键基因.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 硫化 (H2S) 被公认为涉及许多生物过程的气体传递物.
- 它在植物中的作用是复杂的,因为它参与硫代谢和氨酸 (Cys) 合成,在其信号功能中产生模两可.
- 植物内源性H2S生产与Cys代谢有关,该代谢参与各种信号通路.
研究的目的:
- 研究H2S作为气体传递物的不同作用,与植物中Cys合成的基质相比.
- 识别受H2S信号调节的基因,独立于Cys代谢.
- 在植物发展和环境反应中阐明H2S信号网络.
主要方法:
- 植物苗的外源H2S烟雾化和氨酸 (Cys) 处理.
- 测量内生H2S和Cys的产量和含量.
- 综合性转录基因分析 (RNA测序) 来识别差异表达基因 (DEG).
- 基因本体学 (GO) 和基因和基因组 (KEGG) 丰富分析的京都百科全书.
主要成果:
- 无论是H2S烟雾化还是Cys处理,都调节了内源的H2S和Cys水平.
- 转录组分析显示,261个基因对H2S化有反应,其中72个由Cys治疗共同调节.
- 189个基因是由H2S独特调节的,独立于Cys,并通过激素信号转导,植物病原体相互作用,基因生物合成和MAPK信号传递等途径进行丰富.
- 这些受H2S调节的基因通常编码涉及植物发育和应激反应的转录因子,包括Ca2+信号传递.
结论:
- H2S作为一种气体传递剂,可以调节基因表达,与其作为Cys前体的作用截然不同.
- 189个特定于H2S的DEG是H2S信号通路的关键组成部分,独立于Cys.运行.
- 这项研究为植物中的H2S信号网络提供了宝贵的见解,增强了我们对其在发育和应激适应中的作用的理解.
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