在肠内分泌细胞中的表达和酸化
Guillaume Chapelet1, Nora Béguin1, Blandine Castellano2
1Nantes Université, INSERM, CHU Nantes, The Enteric Nervous System in Gut and Brain Disorders, Nantes, France.
Frontiers in neuroscience
|June 19, 2023
概括
这项研究发现,陶蛋白存在于人类肠道肠内分泌细胞 (EEC) 中,并被化. 像 propionate 和 butyrate 这样的短链脂肪酸可以降低这些细胞中 Thr205 的 tau 酸化.
科学领域:
- 神经科学是一个神经科学.
- 胃肠病学 胃肠病学
- 细胞生物学 细胞生物学
背景情况:
- 肠-大脑轴与帕金森病 (PD) 病原发生有关.
- 肠膜中的肠内分泌细胞 (EEC) 越来越多地被认为是它们在PD中的作用.
- α-synuclein和tau蛋白质是神经退行的主要参与者,具有潜在的相互作用.
研究的目的:
- 为了调查人类EEC中tau蛋白的存在,异型形状和酸化状态.
- 探索短链脂肪酸 (SCFA) 对欧共体酸化的影响.
主要方法:
- 在人类结肠样本上使用抗陶和EEC标志物抗体进行免疫组织化学检测.
- 在EEC线路中对tau表达和异型的西部斑块和RT-PCR分析 (GLUTag,NCI-H716).
- 兰巴达酸酶治疗以评估陶酸化和SCFA治疗以评估其对Thr205.5的陶酸化的影响.
主要成果:
- 陶蛋白是从成年人结肠中在EEC表达和酸化的.
- 欧洲经济共同体线主要表达两种tau异型,在基底条件下酸化.
- 酸和丁酸盐治疗降低了GLUTag细胞中Thr205的陶酸化.
结论:
- 这是第一个在人类EEC和EEC线上描述tau的研究.
- 结果表明在EEC功能中的作用以及与病变和同核病变的潜在相关性.
- 为进一步研究在EEC中的功能及其与神经退行性疾病的联系提供了基础.
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