在阿片类药物诱导的便秘大鼠中,通过圣神经刺激调节肠道神经系统
Liyun Wang1, Payam Gharibani2, Yi Yang3,4
1Department of Gastroenterology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
Frontiers in neuroscience
|June 19, 2023
概括
圣神经刺激 (SNS) 通过改善肠道运动性和逆转神经元损伤,有效地治疗小鼠的洛佩拉胺诱导的便秘. 这种疗法可能通过GDNF-PI3K/Akt路径起作用,为便秘管理提供了一种新的方法.
科学领域:
- 胃肠病学 胃肠病学
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 圣神经刺激 (SNS) 用于便秘,但其涉及肠道神经系统 (ENS) 和肠道运动的机制尚不清楚.
- 了解ENS在SNS治疗便秘中的参与,对于优化治疗策略至关重要.
研究的目的:
- 为了研究肠道神经系统 (ENS) 在神经神经刺激 (SNS) 中的作用,用于治疗小鼠模型中的洛佩拉胺诱导的便秘.
- 阐明SNS在恢复肠道运动和神经元功能的基础分子机制.
主要方法:
- 在老鼠中诱导了洛佩拉胺诱导的便秘.
- 每天进行圣神经刺激 (SNS) 或假SNS,持续一周.
- 结肠组织分析包括免疫组织化学和神经元标记物 (ChAT,nNOS,PGP9.5) 和生存因子 (p-AKT,GDNF) 的西部斑.
主要成果:
- 圣神经刺激 (SNS) 显著缩短了结肠过境时间,并解决了洛佩拉胺诱导的便秘,增加了便输出.
- SNS逆转了洛佩拉胺诱导的PGP9.5和CHAT阳性细胞的减少,并使CHAT和nNOS蛋白表达正常化.
- 质神经系统增加了质细胞衍生神经营养因子 (GDNF) 和酸化AKT (p-AKT) 表达,并使阴道活动正常化.
结论:
- 圣神经刺激 (SNS) 有效地改善了小鼠的洛佩拉胺诱导的便秘.
- 显然,SNS可以恢复肠道神经元功能和肠道运动,可能是通过GDNF-PI3K/Akt途径.
- 这些发现表明,SNS是一种有前途的治疗方法,用于阿片类药物引起的便秘.
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