相关实验视频

Updated: Jul 26, 2025

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

血清衍生外体的不同表达的miRNA概况在突然感觉神经听力损失的患者中

Juhong Zhang^1,2, Haizhu Ma¹, Guijun Yang¹

¹Department of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, Chongqing, China.

Frontiers in neurology

|June 19, 2023

概括

这项研究确定了来自突然神经感官听力损失 (SSNHL) 患者的血清外体中的18种差异表达的microRNA (miRNA). 这些外体miRNAs,包括特定的候选人,可以作为SSNHL的潜在生物标志物.

科学领域:

生物化学生物化学
基因组学就是基因组学.
耳鼻喉科耳鼻喉科耳鼻喉科

背景情况:

突然感觉神经听力损失 (SSNHL) 是一种复杂的疾病,基础机制尚不清楚.
外体,小的细胞外囊泡,携带microRNAs (miRNAs) 和涉及细胞间通信和疾病的发病.

研究的目的:

为了比较SSNHL患者和健康对照者之间的血清外体miRNA概况.
为了识别潜在的外体miRNA生物标志物用于SSNHL诊断和理解其病变.

主要方法:

从SSNHL患者和对照组中分离出血清外体.
用纳米粒子跟踪分析,传输电子显微镜和西式涂抹来表征外体.
miRNA转录组测序确定了差异表达的miRNAs (DE-miRNAs),随后进行RT-qPCR验证.

主要成果:

在SSNHL病例中发现了18种外体DE-miRNA (3种上调,15种下调).
功能分析表明参与"蛋白质结合"",金属离子结合"和信号通路,如"Ras"和"Hippo".
特定的miRNAs (PC-5p-38556_39,PC-5p-29163_54,miR-93-3p) 显示出显著的差异表达,测序和RT-qPCR之间具有很高的一致性.

结论:

这项研究确定了与SSNHL相关的18种外体DE-miRNA.
特定的miRNAs,包括PC-5p-38556_39,PC-5p-29163_54和miR-93-3p,作为SSNHL的潜在生物标志物显示出希望.

关键词:

生物标志物生物标志物外基因组是外基因组的组成部分.这是一个小RNARNA.miRNA转录基因组序列的测序突然的感觉神经神经听力损失.

更多相关视频

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

Isolation of Small Noncoding RNAs from Human Serum

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...