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释放的dDNA触发性炎症酶作为肠道辐射保护点

Long Chen^1,2, Ziwen Wang³, Jie Wu¹

¹State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Rocket Force Medicine Army Medical University Chongqing China.

Clinical & translational immunology

|June 19, 2023

概括

放射治疗通过自身dsDNA引发巨细胞中的炎症体引起肠道粘膜炎. 迪苏尔菲拉姆 (DSF) 抑制了这种途径,为辐射引起的肠损伤提供了潜在的治疗方法.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
在瘤学瘤学.

背景情况:

肠道粘膜炎是放射治疗的重要副作用之一.
驱动肠道粘膜炎的特定免疫原体尚未完全理解.
目前仅有有限的辐射保护剂可供使用.

研究的目的:

为了研究双链DNA (dsDNA) 触发的炎症体在辐射诱导的肠粘膜炎中的作用.
为了确定潜在的免疫原体,负责肠道放射性毒性.
探索缓解肠道粘膜炎的治疗策略.

主要方法:

针对促炎性细胞因子的ELISA.
辐射诱导肠道损伤的小鼠模型 (生存率,体重,组织学,屏障功能).
西部斑点,免疫光,共免疫沉和流动细胞测量以分析dSDNA和炎症酶激活.

主要成果:

升高的IL-1β和IL-18与放射治疗患者的腹相关.
被辐射的肠上皮细胞 (IECs) 释放dDNA,作为免疫原体.
dsDNA通过HMGB1/RAGE激活巨细胞中的AIM2炎症酶,增加IL-1β和IL-18.
迪苏尔菲拉姆 (DSF) 通过抑制炎症酶来减轻肠道放射性毒性.

结论:

辐射IECs的细胞外自我dsDNA是一种免疫原,可触发肠道粘膜炎.
向巨细胞中dDNA触发的炎症酶是一种有前途的治疗策略,用于放射治疗的副作用.

关键词:

在AIM2中,AIM2是AIM2.垃圾填埋场是一个垃圾填埋场.这就是HMGB1的特点.这是一种二硫胺.dsDNADNADNA 的意思是什么一些炎症一些炎症肠道辐射毒性肠道辐射毒性

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