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Updated: Jul 26, 2025

Gene Editing of Primary Rhesus Macaque B Cells

Gene Editing of Primary Rhesus Macaque B Cells

Published on: February 10, 2023

一种 SMART 方法,有效地从单个 rhesus macaque 记忆B 细胞中分离单克隆抗体

Jason T Weinfurter¹, Sarah N Bennett¹, Matthew Reynolds^1,2

¹Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison WI.

bioRxiv : the preprint server for biology

|June 19, 2023

概括

一种使用RNA转录的5'端切换机制 (SMART) 的新方法可以放大来自单个 rhesus macaque B 细胞的免疫球蛋白变量 (IgV) 基因. 这种技术改善了针对疫苗和传染病研究的抗原特异性抗体的分离.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
灵长类动物研究研究

背景情况:

在 rhesus macaques (RMs) 中对抗原特异性B细胞进行表征对于疫苗和传染病研究至关重要.
现有的使用5'复合 (MTPX) 起始剂的方法面临着由于领导序列变异性而有效捕获各种RM IgV基因的挑战.

结论:

这种方法增强了从单个RMB细胞中分离抗体,促进了遗传和功能特征.
它在现有的PCR克隆技术上为RM抗体提供了显著的优势.
预计该技术将利用RM模型推进疫苗开发和传染病研究.

关键词:

5 赛车比赛类似免疫缺陷病毒病毒.发现抗体的发现.一个单克隆抗体.rhesus macaques 是一种类型的.普遍的原始发光器是普遍的原始发光器

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