移动遗传元素及其相关宿主在复杂微生物组中的空间映射
Benjamin Grodner1, Hao Shi1, Owen Farchione1
1Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
研究人员开发了一种新的成像技术,用于绘制人类微生物群中的移动遗传元素 (MGE) 和它们的细菌宿主. 这种方法揭示了MGEs在特定的利基中聚集,可能导致抗菌素耐药性的传播.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- 移动遗传元素 (MGE) 推动细菌群体中抗菌素耐药性等特征的传播.
- 了解MGE的空间分布和宿主协会至关重要,但具有挑战性.
- 现有的工具缺乏在复杂的微生物环境中绘制MGEs的分辨率.
研究的目的:
- 开发和验证一种新的成像方法,用于同时可视化MGEs和宿主细菌.
- 在人类口服生物膜中空间绘制抗菌素耐药性 (AMR) 质体和细菌菌体的地图.
- 调查MGEs的空间分布模式,并确定它们的细菌宿主.
主要方法:
- 结合单分子DNA光在现场混合 (FISH) 与多重核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核酸核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核酸核糖核糖核糖核糖核酸核糖核糖核糖核酸核糖核糖核糖核糖核糖核糖核糖核糖核酸核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核糖核酸核糖核糖核糖核酸核酸核酸核酸核酸核酸核酸核酸核酸核酸核酸核酸
- 应用了该技术来分析人类口腔生物膜.
- 空间映射的细菌体和AMR等离子体,识别宿主种类.
主要成果:
- 现场成功地将MGEs与宿主细菌种群一起可视化和绘制地图.
- 在口服生物膜中观察到AMR等离子体和prophages的明显集群.
- 证明了识别MGEs特定细菌宿主的能力.
结论:
- 开发的成像方法使微生物群落中MGEs的详细空间分析成为可能.
- MGEs,包括AMR等离子体和菌体,在生物膜中形成独特的空间.
- 这些发现表明,专门的利基促进了MGE维护和水平基因转移,影响了抗菌素耐药性的动态.
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