一个有义务的细胞内细菌病原体形成了直接的,跨王国膜接触点
Yamilex Acevedo-Sánchez1, Patrick J Woida1, Stephan Kraemer2
1Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
里克特西亚帕克里细菌形成与宿主细胞内质网膜 (ER) 的直接膜接触点. 这些王国间的接触模仿了有机体-ER相互作用,揭示了新的病原体-宿主通信.
科学领域:
- 细胞生物学 细胞生物学
- 微生物学 微生物学
- 病原体与宿主相互作用
背景情况:
- 细胞平衡依赖于通过膜接触点 (MCS) 的器官间通信.
- 已知细胞内病原体可以操纵真核MCS,但直接的原核-真核MCS没有被表征.
研究的目的:
- 研究细胞内细菌Rickettsia parkeri及其宿主细胞之间的膜接触点的存在和性质.
- 阐明这些跨王国膜相互作用背后的分子机制.
主要方法:
- 活细胞显微镜 活细胞显微镜
- 传输电子显微镜 (TEM) 的使用
- 聚焦离子束扫描电子显微镜 (FIB-SEM).
- 主体结合蛋白 (VAPA,VAPB) 的耗尽
主要成果:
- 里克特西亚帕克里在它的外膜和宿主细胞的粗内 плазма网膜 (ER) 之间形成了直接的膜接触点.
- 这些接触器具有距离约55纳米的绳索.
- 随着ER特异性绳索VAPA和VAPB的耗尽,Rickettsia-ER接触的频率显著降低.
- 观察到的瑞克特西亚-ER接触似乎模仿已建立的有机体-ER MCSs.
结论:
- 里克特西亚帕克里 (Rickettsia parkeri) 与宿主ER建立了一个独特的,直接的王国间膜接触点.
- 主体ER绑定蛋白 (VAPA,VAPB) 可能在调解这些病原体与宿主相互作用方面发挥作用.
- 这些发现揭示了病原体与宿主通信的新机制,并扩大了已知的膜接触部位的范围.
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