葡萄糖新陈代谢的时空空间分类指导哺乳动物胃流动的进展
Dominica Cao1, Liangwen Zhong1, Anupama Hemalatha1
1Department of Genetics, Yale School of Medicine, Yale University, New Haven, CT 06520.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
胚胎在胃流动过程中使用葡萄糖的方式不同. 细胞命运的获取依赖于赫索胺生物合成途径 (HBP),而中皮迁移则使用糖解,揭示了代谢对发育的控制.
科学领域:
- 发展生物学 发展生物学
- 代谢调节 代谢调节 代谢调节
- 胚胎发生是胚胎发生.
背景情况:
- 消化是一个关键的发育阶段,需要显著的代谢支持.
- 在胃流动期间葡萄糖代谢的空间映射仍然不太了解.
- 了解代谢变化是理解胚胎发育和相关疾病的关键.
研究的目的:
- 为了研究小鼠胃流动期间葡萄糖代谢是如何在空间上调节的.
- 确定胃流动期间参与不同细胞过程的特定代谢途径.
- 探索代谢途径与胚胎发生过程中的生长因子信号之间的相互作用.
主要方法:
- 对小鼠胚胎进行定量实时成像.
- 在体外干细胞分化模型.
- 胚胎衍生的组织扩展培养.
- 葡萄糖代谢的机制研究.
主要成果:
- 在胃流动过程中,葡萄糖通过不同的代谢途径 (HBP和糖解) 以细胞类型和阶段特定的方式被利用.
- 细胞命运的获取和上皮转化为介质细胞的过渡 (EMT) 取决于胺生物合成途径 (HBP).
- 新形成的中皮的迁移和扩张需要糖解.
- 代谢调节与纤维细胞生长因子 (FGF) 信号协调.
结论:
- 葡萄糖代谢在胃流动期间指导胚胎形态发生过程中起着独特的作用.
- 葡萄糖代谢的特定分支对于不同的细胞事件至关重要,如细胞命运决定和细胞迁移.
- 代谢和FGF信号之间的交叉对胃化进展至关重要.
- 这些发现提供了对发育障碍,癌症和先天性疾病的见解.
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