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局部特异的人体内源逆转录病毒揭示了新的淋巴瘤亚型

Bhavya Singh¹, Nicholas Dopkins¹, Tongyi Fei¹

¹Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, USA.

bioRxiv : the preprint server for biology

|June 19, 2023

概括

可移植元素 (TE),包括人体内源逆转录病毒 (HERV),在B细胞和淋巴瘤中表现出独特的表达模式. 这些签名可以改善B细胞淋巴瘤的分类和诊断.

科学领域:

基因组学就是基因组学.
癌症生物学癌症生物学
免疫学免疫学免疫学

背景情况:

癌症异质性源于各种瘤源机制,包括细胞起源,突变发生和病毒感染.
传统上,B细胞淋巴瘤的分类考虑了这些因素,但可移植元素 (TE) 的作用仍未得到充分研究.
试管婴儿是移动的遗传元素,在瘤发生和细胞识别中具有潜在的作用.

研究的目的:

研究B细胞淋巴瘤中TEs的表达和贡献.
确定TE特征是否可以提高B细胞恶性瘤的分类和理解.
在淋巴瘤研究中探索逆转录核分析的潜力.

主要方法:

TE表达的综合性,位置特异性表征.
在良性生殖中心 (GC) B细胞中TE表达的分析.
在扩散性大B细胞淋巴瘤 (DLBCL),爱斯坦-巴尔病毒 (EBV) 阳性和EBV阴性伯基特淋巴瘤 (BL) 和卵泡淋巴瘤 (FL) 中进行了比较分析.

主要成果:

在GC B细胞和各种淋巴瘤亚型中显示出独特的人体内源逆转录病毒 (HERV) 签名.
展示了HERV活性与基因表达相结合,可以在淋巴细胞恶性瘤中定义B细胞系.
确定了针对每个B细胞和淋巴瘤类型的特异性TE表达特征.

结论:

TE签名,特别是HERVs,为B细胞身份提供了一种新的信息层.
复原转录组分析具有改善淋巴瘤分类和诊断的巨大潜力.
TE表达模式可能有助于识别新的治疗点和患者子组.

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