一个不成熟的人体内源性逆转录病毒的分子结构和保护
Anna-Sophia Krebs1, Hsuan-Fu Liu2, Ye Zhou3
1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, OX3 7BN, UK.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
人体内源性逆转录病毒K (HERV-K) 的结构是使用冷电子断层扫描揭示的. 这项研究详细介绍了HERV-K.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 基因组学就是基因组学.
背景情况:
- 人体内源逆转录病毒 (HERVs) 占人类基因组的很大一部分.
- 人体内源逆转录病毒K (HERV-K) 是最近获得的HERV,与癌症,肌缩性侧面硬化和衰老有关.
研究的目的:
- 使用冷电子断层扫描和亚断层扫描平均值,阐明未成熟的人体内源性逆转录病毒K (HERV-K) 的分子结构.
- 将HERV-K体结构与其他逆转录病毒进行比较,以了解保存的组装和成熟机制.
主要方法:
- 使用冷电子断层扫描和亚断层扫描平均值 (cryoET STA) 来从原生病毒样颗粒 (VLPs) 确定不成熟的HERV-K的结构.
- 用全原子分子动力学模拟和突变研究来详细描述囊蛋白相互作用.
主要成果:
- 化ET STA 地图揭示了3.2 Å分辨率的未成熟的HERV-K体结构,其中包括由6螺旋束稳定的六合体单位.
- 由于额外的SP1和p15,HERV-K VLP在病毒膜和囊网之间表现出更大的距离.
- 囊蛋白在成熟过程中发生显著的形状变化,类似于HIV-1.
结论:
- 这项研究提供了对未成熟的HERV-K体组件的高分辨率结构洞察.
- HERV-K 组装和成熟与其他逆转录病毒共享保存机制,突出了进化保存.
- 了解HERV-K的结构可能有助于了解它在疾病和衰老中的作用.
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