跨越mRNA生命周期的RNA结合蛋白相互作用体的大规模地图
Lena Street1,2, Katherine Rothamel1,3,4, Kristopher Brannan1,3,4,5,6
1These authors contributed equally.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
这项研究绘制了RNA结合蛋白 (RBPs) 在整个信使RNA (mRNA) 生命周期中使用的蛋白质相互作用图. 这些发现揭示了这些蛋白质网络如何调节RNA处理,并识别了参与多个mRNA阶段的蛋白质.
科学领域:
- * 分子生物学 * 分子生物学
- * 基因组学 是一个学科.
- * 蛋白质组学 蛋白质组学
背景情况:
- *信使RNAs (mRNAs) 与RNA结合蛋白 (RBPs) 相结合,形成核糖核蛋白复合体 (RNP),对RNA处理和成熟至关重要.
- *研究主要集中在蛋白质-RNA相互作用上,对蛋白质-蛋白质相互作用 (PPI) 的重点较少,以了解蛋白质在mRNA生命周期阶段的作用.
研究的目的:
- * 在整个mRNA生命周期中构建一个RNA意识的,以RBP为中心的PPI地图.
- * 识别和描述参与mRNA处理和成熟的各个阶段的蛋白质的作用.
- * 探索RBP复合体的动态性质及其通过RNA存在的调节.
主要方法:
- * 在大约100个内源性RBP上进行了免疫净化与质谱学 (IP-MS) 相结合.
- * 利用大小排除色谱与质谱学 (SEC-MS) 结合分析蛋白质复合体.
- * 在RNase的存在和缺席下研究RBP相互作用,以评估RNA依赖相互作用.
主要成果:
- *生成了一个全面的PPI地图,证实了已知的8700个,并在1125种蛋白质中发现了20359种新的相互作用.
- *确定了73%的已识别的蛋白相互作用是由RNA的存在调节的.
- *揭示了近一半的研究蛋白质参与至少两个不同的mRNA生命周期阶段.
- *确定了特定的蛋白质,如ERH和SNRNP200,参与多个RNA处理事件和应激反应.
结论:
- *开发的以RBP为重点的PPI网络是发现多阶段RBP的宝贵资源.
- * 提供了整个mRNA成熟过程中RBP复合体的组成和功能的见解.
- *强调RNA在调节RNP中的蛋白质-蛋白质相互作用方面的重要作用.
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