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跨代表观遗传MHC I类基因表达受CCAAT促销元件的调节
Jocelyn D Weissman1, Aparna Kotekar1, Zohar Barbash1
1Experimental Immunology Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
CCAAT盒子促进元件调节稳定的跨代表观遗传. 它的突变破坏了几代人的基因表达,为保持基因表达忠实提供了洞察力.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 跨代表观遗传传递的特征跨代没有DNA序列变化.
- 分子机制包括基因素和DNA的修改,以及非编码RNA.
- 以前的研究记录了各种生物体在压力或代谢变化下表观遗传的遗传.
研究的目的:
- 研究CCAAT盒子促进元在稳定的跨代表观遗传中的作用.
- 了解促进子元素的突变如何影响跨代基因表达的忠实性.
主要方法:
- 在一个MHC I类转基因中产生了具有突变CCAAT盒子元素的转基因线.
- 分析了基因表达模式,基因素修饰,RNA聚合酶II结合,DNA甲基化和跨代核细胞的占用率.
- 评估了NF-Y结合,CTCF结合和与基因表达状态相关的DNA循环模式.
主要成果:
- CCAAT盒子的突变导致了至少四代后代的多变基因表达.
- 基因素修饰和RNA polII结合与表达水平相关,而DNA甲基化和核细胞占用没有.
- CCAAT盒子突变取消了NF-Y结合,改变了CTCF结合,并改变了DNA循环,与遗传表达状态相关联.
结论:
- 已确定CCAAT促销元件是稳定的跨代表观遗传的一个关键调节器.
- 研究结果表明,CCAAT盒子突变通过改变转录因子结合和染色质结构来影响表观遗传.
- 这项研究提供了对跨代保持基因表达忠实性的见解,与含有CCAAT盒的30%的真核细胞促进体有关.
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