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交易acyls和交换糖:在三合体中的代谢创新

Paul D Fiesel¹, Rachel E Kerwin¹, A Daniel Jones¹

¹Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48823 USA.

bioRxiv : the preprint server for biology

|June 19, 2023

概括

这项研究揭示了Solanum属的多样化的乙糖结构,广泛发现的基于伊诺西的新型化合物. 一个新的酶,Solanum melongena AcylSugar AcylTransferase 3-Like 1 (SmASAT3-L1),被确定,提供了关于糖演变的见解.

科学领域:

植物生物化学植物生物化学
代谢学代谢学代谢学
基因组学就是基因组学.

背景情况:

科植物种类产生各种各样的专门代谢物,包括保护性酸糖.
之前的研究发现了葡萄糖和糖核在酸糖中,在Solanum属中进行的分析有限.

研究的目的:

为了探索Solanum亚类的糖的化学多样性.
识别新型的乙糖结构和负责它们合成的酶.

主要方法:

来自Solanum melongena的乙烯酸糖的深入表征.
液态染色学-质谱学 (LC-MS) 对31种Solanum物种的分析.
对组织特异性转录组和酶识别的分析.

主要成果:

在Solanum melongena中识别了八种不寻常的内醇或内醇糖化物核心酸糖.
在Solanum中揭示了惊人的酸糖多样性,其中酸醇广泛存在,而酸葡萄糖/糖更受限制.
发现了Solanum melongena AcylSugar AcylTransferase 3-Like 1 (SmASAT3-L1),这是一个功能分离的酶.

结论:

索拉努姆表现出显著的酸糖化学多样性,在种类和物种之间有明显的模式.
SmASAT3-L1代表了一种新型的AcylSugar AcylTransferase类,与ASAT4类酶不同.
这项研究为了解乙糖的演变和在育种和合成生物学中的应用奠定了基础.

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