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Updated: Jul 26, 2025

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快速和多重复合核酸检测使用可编程的基于aptamer的RNA开关.

Zhaoqing Yan1,2,3,4, Anli A Tang5,6,4, Amit Eshed1,3

  • 1Department of Biomedical Engineering, Boston University, Boston, MA, USA.

medRxiv : the preprint server for health sciences
|June 19, 2023
PubMed
概括

新的Aptaswitch提供了快速,低成本的传染病检测. 这些RNA开关为核酸检测提供快速的视觉光读数,使得早期诊断成为可能.

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科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断
  • 核酸化学的核酸化学

背景情况:

  • 快速,简单和低成本的诊断技术对于传染病控制至关重要.
  • 现有的方法往往缺乏速度,简单性或可供广泛使用的可负担性.

研究的目的:

  • 开发一种新的类型的基于aptamer的RNA开关,称为aptaswitches,用于核酸检测.
  • 为了证明用于诊断应用的适应开关的多功能性和灵敏性.

主要方法:

  • 设计的aptaswitches启动的记者aptamer折叠在绑定特定的目标核酸.
  • 用了六种不同的光aptamer/fluorogen对进行记者读取.
  • 集成的aptaswitches与同热放大,以提高一个反应的灵敏度.

主要成果:

  • Aptaswitches提供快速 (只需5分钟) 和强烈的光读数,可通过眼睛检测.
  • 证明了多重检测与各种记者颜色.
  • 通过同热放大和适应开关实现了降至1RNA拷贝/μL的灵敏度.
  • 在临床唾液样本中成功检测到SARS-CoV-2,精度为96.67%,在30分钟内.

结论:

  • Aptaswitch是用于核酸检测的多功能工具,可适应各种目标和报告系统.
  • 该技术可实现快速,灵敏和可视检测的诊断.
  • 应用开关可以很容易地集成到对传染病的诊断测试中.