在芽和裂变酵母中量化流压力,基于透性质
1Department of Cell and Tissue Biology, University of San Francisco, CA, USA.
bioRxiv : the preprint server for biology
|June 19, 2023
概括
测量像酵母这样的围墙细胞中的压是具有挑战性的. 这项研究引入了一种基于原生质的新方法,用于准确量化各种酵母物种的压,揭示了明显的菌株特异性差异.
科学领域:
- 细胞生物物理学 细胞生物物理学
- 微生物学 微生物学
- 定量生物学的定量生物学.
背景情况:
- 包括酵母在内的围墙细胞,利用压来生长和形成.
- 精确测量酵母中的压仍然是一个重大挑战.
研究的目的:
- 开发一种简单,强大的方法来量化酵母中的压.
- 为了比较不同酵母物种和菌株的压.
主要方法:
- 使用酵母原生菌作为氧度计来确定同度.
- 采用了三种不同的方法 (细胞体积,光体强度,纳米rheology) 来识别同位素条件.
- 在 *S. pombe*, *S. japonicus* 和两个 *S. cerevisiae* 菌株中测量压.
主要成果:
- 提供了 *S. pombe* (1.0 ± 0.1 MPa), *S. japonicus* (0.49 ± 0.01 MPa), *S. cerevisiae* W303a (0.5 ± 0.1 MPa) 和 *S. cerevisiae* BY4741 (0.31 ± 0.03 MPa) 的定量压值.
- 在压和纳米rheology,即使在野生型 *S. cerevisiae* 菌株之间,也显示出显著的差异.
- 在三种独立的同位素点确定方法中验证一致性.
结论:
- 开发的原生细胞度仪方法提供了一种可靠的方法来测量酵母压.
- 突出了酵母物种内显著的生物物理异质性,影响细胞力学.
- 为细胞力学和进化生物学中的比较研究提供了关键数据.
关键词:
这种植物是Saccharomyces cerevisiae.这种植物是Schizosaccharomyces pombe.这种病名是:schizosaccharomyces. 在日本,日本人就是日本人.细胞体积细胞体积的细胞体积.细胞质的拥挤 细胞质拥挤光是一种光.一个原生质原生质.图尔戈尔的压力是压力.酵母酵母是一种酵母.更多相关视频
11:18Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
Published on: July 15, 2019
11.6K
14:20Measuring the Osmotic Water Permeability Coefficient Pf of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
16.1K
相关概念视频
02:40Osmosis and Osmotic Pressure of Solutions
40.5K
A number of natural and synthetic materials exhibit selective permeation, meaning that only molecules or ions of a certain size, shape, polarity, charge, and so forth, are capable of passing through (permeating) the material. Biological cell membranes provide elegant examples of selective permeation in nature, while dialysis tubing used to remove metabolic wastes from blood is a more simplistic technological example. Regardless of how they may be fabricated, these materials are generally...
40.5K
01:28Factors Influencing Microbial Growth: Osmolarity
49
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
49
01:20Tonicity in Plants
30.8K
Plant cells maintain appropriate osmotic balance in extreme conditions. For instance, plants in dry environments store water in vacuoles, limit the opening of their stoma, and have thick, waxy cuticles to prevent unnecessary water loss. Some species of plants that live in salty environments store salt in their roots. As a result, water osmosis occurs in the root from the surrounding soil.
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
30.8K
01:27Microbial Growth Measurement: Indirect Methods
80
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
80
01:16Tonicity in Animals
3.2K
Tonicity describes the amount of solute in a solution. The measure of the tonicity of a solution, or the total amount of solutes dissolved in a specific amount of solution, is called its osmolarity. Three terms—hypotonic, isotonic, and hypertonic—are used to relate the osmolarity of a cell to the osmolarity of the extracellular fluid that contains the cells. In a hypotonic solution, such as tap water, the extracellular fluid has a lower concentration of solutes than the fluid inside...
3.2K
01:23Microbial Growth Measurement: Direct Methods
84
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
84