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由miR-146b-3p基因下调的PTPN12会影响喉状细胞癌的恶性进展

Lili Lan^1,2, Huan Cao¹, Lei Zhao¹

¹Otolaryngology Head and Neck Surgery Department, The Second Hospital of Hebei Medical University, Shijiazhuang 050005, Hebei, China.

Open medicine (Warsaw, Poland)

|June 19, 2023

概括

这项研究表明,喉状状细胞癌 (LSCC) 中蛋白氨酸酸酶非受体12型 (PTPN12) 表达的减少促进了瘤的生长. 确定了miR-146b-3p/PTPN12轴作为LSCC的潜在治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

喉平细胞癌 (LSCC) 是一种重要的头癌.
蛋白氨酸酸酶非受体12型 (PTPN12) 作为瘤抑制剂的作用已在各种癌症中得到证实,但其在LSCC中的特定功能和调节仍然不清楚.
确定新的生物标志物和治疗点对于改善LSCC治疗结果至关重要.

研究的目的:

研究LSCC中PTPN12的表达水平和调节机制.
阐明miR-146b-3p在调节PTPN12表达中的作用及其对LSCC进展的影响.
探索miR-146b-3p/PTPN12轴作为LSCC的治疗点的潜力.

主要方法:

使用定量实时RT-PCR (qRT-PCR),西斑 (WB) 和免疫组织化学分析PTPN12和miR-146b-3p表达.
进行了体外测试 (扩散,迁移,入侵),以评估PTPN12和miR-146b-3p的功能作用.
采用双 luciferase 记者基因测试来确认 miR-146b-3p 和 PTPN12 之间的直接向关系.
生物信息学工具被用来预测和验证PTPN12的下游目标基因,包括EGFR和ERBB2.

主要成果:

与正常邻近组织相比,LSCC组织中PTPN12mRNA和蛋白质表达显著下降.
较低的PTPN12表达与较差的病理差异化和LSCC中高级TNM阶段相关.
过度表达PTPN12抑制了LSCC细胞的增殖,迁移和入侵,而高表达miR-146b-3p促进了这些过程.
miR-146b-3p直接准并抑制PTPN12,这个轴调节LSCC细胞的行为.
PTPN12对EGFR表达有负面调节,而miR-146b-3p对EGFR表达有积极调节. 无论是PTPN12还是miR-146b-3p都会影响ERBB2的表达.

结论:

在LSCC中,PTPN12通过抑制增殖,迁移和入侵而起到瘤抑制作用.
在LSCC的发展和进展中,miR-146b-3p/PTPN12轴起着至关重要的作用.
向miR-146b-3p/PTPN12通路为喉状细胞癌提供了一个有希望的治疗策略.

关键词:

喉皮状细胞癌的癌症.在PTPN12中,这是入侵的入侵.在 miR-146b-3p 中使用.移民移民迁移迁移

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