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基于纤维素组合的β-氨酸合成大肠杆菌的构造技术研究

Jie Lu^1,2, Guodong Wang^1,2, Cuiping Yang^1,2

¹State Key Laboratory of Biobased Material and Green Papermaking (LBMP), Qilu University of Technology, Jinan, Shandong, China.

Frontiers in bioengineering and biotechnology

|June 19, 2023

概括

工程Escherichia coli通过阻断L-lysine通路和使用纤维素体技术,通过微生物发酵有效地产生β-alanine. 这种生物合成方法可显著增加工业应用中的β-氨酸产量和含量.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
酶工程是什么? 酶工程是什么?

背景情况:

β-氨酸是一种有价值的化合物,用于食品,医药和工业.
传统的β-氨酸生产方法会造成污染.
微生物发酵和酶催化提供了绿色,高产的替代品.

研究的目的:

从葡萄糖中设计一种大肠杆菌菌株以有效地生产β-氨酸.
使用纤维素体技术提高催化和产品合成效率.
通过路径修改和酶组合来增加β-氨酸的产量和含量.

主要方法:

通过在L-lysine生产器中敲除阿斯巴酸激酶基因 (lysC) 来构建一个重组的大肠杆菌菌株.
利用基因编辑阻止L-lysine合成途径,将代谢物重定向到β-alanine.
组装了关键酶,L-阿斯巴酸-α-脱碳酶 (bspanD) 和阿斯巴酸氨基转移酶 (aspC),与纤维素组分 (多克林和凝聚素) 以提高催化效率.

主要成果:

阻止L-lysine通路减少了副产品和增加了β-alanine产量.
两种酶方法和纤维素组合显著提高了催化效率和酶表达.
工程菌株的β-氨酸产量达到7.439 mg/L和25.87 mg/L,最终含量为755.465 mg/L,在5L发酵器中.
合成的β-氨酸含量是10.47和36.42倍高,与没有组装的细胞体的菌株相比.

结论:

工程化大肠杆菌菌株显示出一种高效的生物合成途径,用于生产β-氨酸.
基于纤维素体的多酶自组装系统有效地增强了β-氨酸的酶性生产.
这项研究为使用可持续生物合成方法进行工业规模的β-氨酸酶化生产提供了基础.

关键词:

纤维素体细胞体是什么?凝聚力在凝聚力中在船里,他做了一些工作.酶催化酶的催化作用基因淘汰赛基因淘汰赛这是一种β-氨酸 (β-alanine).

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