带有截断的NdhM子单元的NDH-1L在蓝藻细菌的压力条件下不稳定
Liping Che1, Yuecheng Guo1, Yanjie Huang1
1Development Center of Plant Germplasm Resources, College of Life Sciences Shanghai Normal University Shanghai China.
Plant direct
|June 19, 2023
概括
菌NdhM蛋白对于NDH-1L复合物的稳定性至关重要. 切断NdhM削弱了它的相互作用,使NDH-1复合物在压力条件下变得不稳定.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 光合作用研究研究 光合作用研究
背景情况:
- NDH-1复合体对于蓝藻细菌的氧光合作用至关重要.
- NdhM是形成大型NDH-1L复合物的重要子单元.
- 之前的结构研究揭示了NhM的N端β片和中端/C端α螺旋.
研究的目的:
- 研究NdhM的C端α螺旋在NDH-1复合体形成和稳定中的作用.
- 为了描述表达C端截断的NdhM子单元 (NdhMΔC) 的突变蓝藻细菌.
主要方法:
- 在 *Synechocystis* 6803.3 中产生一个NdhMΔC突变.
- 在正常和应激条件下评估NDH-1复合物的积累和活性.
- 免疫块分析以评估NDH-1L水友臂的组装.
主要成果:
- 在标准生长条件下,NdhMΔC突变显示出正常的NDH-1积累和活性.
- 带有截断的NdhM (NdhMΔC) 的NDH-1复合体在压力下表现出不稳定性.
- 在NdhMΔC突变体中,NDH-1L水友性臂的组装不受影响,即使在高温下也是如此.
结论:
- 即使没有C端α螺旋,NdhM也可以与NDH-1复合体结合.
- NdhM的C端α螺旋体有助于NDH-1相互作用的稳定性.
- 截断的NdhM会导致较不稳定的NDH-1L复合体,特别敏感于环境压力.
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