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格雷林不会影响小鼠对复发性低血糖的反调节反应
Kripa Shankar1, Salil Varshney1, Deepali Gupta1
1Center for Hypothalamic Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States.
Frontiers in endocrinology
|June 19, 2023
概括
复发性低血糖症不会改变格林的降低,格林不会影响血糖或对抗调节性激素反应在小鼠与低血糖相关的自主性衰竭 (HAAF). 这一发现澄清了格林在HAAF中的作用.
科学领域:
- 内分泌学 在内分泌学.
- 代谢过程中的代谢.
- 神经科学是一个神经科学.
背景情况:
- 低血糖相关的自主性衰竭 (HAAF) 损害了对糖尿病低血糖的逆调节反应 (CRR).
- 在HAAF背后的分子机制,特别是 ghrelin 的作用,尚未完全理解.
- 以前的研究表明,格林对正常的CRR至小鼠低血糖症至关重要.
研究的目的:
- 调查是否减弱的 ghrelin 释放是 HAAF 的结果.
- 为了确定降低林是否有助于HAAF.
- 为了测试这种假设,格林在HAAF中观察到的减弱的CRR中起作用.
主要方法:
- 使用了C57BL/6N小鼠,激素淘汰 (KO) 小鼠和激素细胞选择性胰岛素受体淘汰 (GhIRKO) 小鼠.
- 这些组接受了euglycemia,单次胰岛素诱导的低血糖症 (1X Hypo) 或复发性胰岛素诱导的低血糖症 (复发性低血糖症).
- 测量血糖水平和反调节激素 (葡萄糖,上腺素) 的血水平.
主要成果:
- 反复出现的低血糖症加剧了血糖降低和减弱的CRR激素升高,与单一事件相比.
- 血激林水平在1XHypo和复发性Hypo组中同样降低.
- 格雷林KO和Ghirko小鼠没有显示过度低血糖或进一步减弱的CRR,表明格雷林不会影响HAAF中的这些结果.
结论:
- 在胰岛素诱导的低血糖症后,血格林的降低不受复发的影响.
- 格雷林在复发性低血糖症期间不会影响血糖水平或削弱CRR激素反应.
- 这些发现表明,格林在这种小鼠模型中不会对HAAF的病理生理学产生影响.
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