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鉴定EGPA和严重的eosinophilic喘中的自身抗原及其潜在的翻译后修饰
Ilaria Esposito1, Ioanna Kontra1, Chiara Giacomassi1,2
1William Harvey Research Institute, Queen Mary University of London, London, United Kingdom.
Frontiers in immunology
|June 19, 2023
概括
这项研究研究了严重酸性喘 (SEA) 的自身抗体,发现与对照组相比,SEA患者的某些自身抗体如原-V和TREM1的水平升高,这表明改善诊断测试的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 肺部病理学 肺部病理学
- 这是一种自身免疫力.
背景情况:
- 严重的酸性喘 (SEA) 呈现慢性气道炎症,暗示可能的自身免疫基础.
- 类似于ANCA相关血管炎的自身抗体可能在SEA病变发生过程中发挥作用.
- 氧化后翻译性修饰 (oxPTM) 可以触发自身抗体反应,但它们在SEA中的作用尚未研究.
研究的目的:
- 在患有严重酸性喘 (SEA) 的患者中调查自身抗体反应.
- 探索潜在的自身抗原,包括那些通过氧化后翻译修饰 (oxPTM) 修饰的抗原.
- 评估SEA.中自抗体血清学的诊断潜力.
主要方法:
- 分析了患有多炎 (EGPA),SEA和健康对照患者的乙酸性颗粒瘤病的血清.
- 采用一种自抗原无定性方法,使用免疫光在受刺激的中性粒细胞和异氨基粒细胞上.
- 一种向自身抗原方法涉及间接ELISA检测对候选蛋白质 (MPO,EPX,原蛋白-V,TREM1,IL1R2) 的原生和oxPTM形式的自身抗体.
主要成果:
- 来自SEA患者的血清显示IgG染色与接受NETosis的中性粒细胞.
- 对原-V,MPO和TREM1的自身抗体在SEA患者中比对照患者的比例更高.
- 在健康和SEA参与者中,对EPX的自身抗体普遍存在;oxPTM没有增加自身抗体检测率.
结论:
- 虽然没有单一的点蛋白对SEA表现出高敏感性,但很大一部分SEA患者至少具有一个自身抗体.
- 这些发现突显了自抗体血清学在改善严重喘诊断策略方面的潜力.
- 对自身抗体概况的进一步研究可能会提高严重酸性喘的诊断准确度.
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