扩展的茎区域的融合行为来自葡萄球菌表面蛋白质,其序列模式差异很大
Alexander E Yarawsky1, Andrea L Ori1,2, Lance R English3
1Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.
Protein science : a publication of the Protein Society
|June 19, 2023
概括
葡萄球菌细胞壁固的蛋白质抵抗紧缩,保持对生物膜形成至关重要的扩展形状. 这种在不同类型的葡萄球菌中保存的功能突显了细菌粘附和感染的关键机制.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 葡萄球菌表皮菌和金黄色葡萄球菌是重要的医院获得的病原体.
- 由这些细菌形成的细菌生物膜抵抗抗生素,并导致持久性感染.
- 细胞壁固 (CWA) 蛋白质对于生物膜发育和细菌病原体产生至关重要.
研究的目的:
- 调查Staphylococcus epidermidis积聚相关蛋白 (Aap) 茎区域中观察到的扩展形状是否是各种葡萄球菌CWA蛋白中保存的特征.
- 描述溶液中葡萄球菌CWA蛋白茎区域的结构性质.
主要方法:
- 循环二重化 (CD) 光谱法,以评估随温度和辅溶剂的二次结构变化.
- 沉速度分析超离心法 (SV-AUC) 来确定溶液的行为.
- 尺寸排除色谱 (SEC) 和小角度X射线散射 (SAXS) 用于结构特征.
主要成果:
- 所有测试的葡萄球菌CWA蛋白茎区域都本质上是无序的,表现出随机的线圈和聚烯II型螺旋结构.
- 这些内在无序的茎区域在溶液中始终采用并保持高度扩展的形状.
- SdrC的Ser-Asp二重复区域显示出与Aap茎区域的功能和结构相似性,尽管有序列分歧.
结论:
- 葡萄球菌CWA蛋白茎区域共享一种保留的功能性质,即抵抗紧缩并保持延伸.
- 这种扩展的形状可能是投射粘接域远离细菌细胞表面的必要条件.
- 这些发现揭示了葡萄球菌CWA蛋白的保存机制,有助于生物膜形成和感染策略.
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