Ptr1和ZAR1免疫受体赋予重叠和不同的细菌病原体效应特异性
Ye Jin Ahn1, Haseong Kim1,2, Sera Choi1
1Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, Korea.
The New phytologist
|June 19, 2023
概括
植物免疫受体,核酸结合和氨酸丰富的重复受体 (NLRs),可以间接地识别病原体作用体. 这项研究确定了Nicotiana benthamiana中的特定NLRs,可以检测针对RIN4的效应因子,揭示了植物免疫力的融合进化.
科学领域:
- 植物免疫学 植物免疫学
- 分子植物病原体相互作用
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 核酸结合和氨酸丰富的重复受体 (NLR) 是关键的植物免疫受体.
- 一些NLR通过监测宿主蛋白点来间接检测病原体效应物.
- 在Arabidopsis thaliana中,RIN4是多个无关效应因子的目标,激活RPM1和RPS2介导的免疫力.
研究的目的:
- 为了识别能识别Arabidopsis RIN4向作用因子的尼科蒂亚娜塔米亚纳NLRs (NbNLRs).
- 通过已识别的NbNLR来研究效应器识别的机制.
主要方法:
- 使用NbNLR病毒诱导基因沉默 (VIGS) 库进行快速反向基因选.
- 通过效应因子识别试验对已识别的NbNLR进行功能性表征.
- 分析RLCK XII家族蛋白JIM2在NbZAR1-介导识别中的作用.
主要成果:
- 鉴定出Ptr1 (NbPtr1) 的尼科蒂亚纳塔米亚纳同类物能够识别伪杆菌效应体AvrRpt2,AvrRpm1和AvrB.
- 鉴别Xanthomonas效应器AvrBsT和Pseudomonas效应器HopZ5是由NbPtr1和NbZAR1.1独立调解的.
- RLCK XII家族蛋白质JIM2对于NbZAR1依赖的AvrBsT和HopZ5.5的识别至关重要.
结论:
- NbPtr1和NbZAR1提供了在识别与序列无关的病原体作用因子方面的融合进化的例子.
- 在Nicotiana benthamiana和Capsicum annuum之间观察到Ptr1和ZAR1在效应因子识别方面的不平等贡献.
- 了解这些NLR介导的免疫机制可以揭示植物扩展效应器识别的新策略.
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