基于中金BODIPY的长波长红外线线粒体准光探测器具有高光稳定性
Shenghe Jiao1, Xiaochun Dong1, Weili Zhao1,2
1School of Pharmacy, Fudan University, Shanghai 201203, PR China. xcdong@fudan.edu.cn.
Analytical methods : advancing methods and applications
|June 19, 2023
概括
研究人员开发了新的深红色光探针用于线粒体. 这些基于BODIPY的探测器比深红色的Mito-tracker更具光稳定性和更少的毒性,提供了改进的长期线粒体跟踪.
科学领域:
- 化学生物学 化学生物学
- 生物物理化学 生物物理化学
- 细胞生物学 细胞生物学
背景情况:
- 线粒追踪深红色 (MTDR) 是一种常见的线粒体探针,但患有光漂白.
- 发展光稳定,长波长光探针对于先进的细胞成像至关重要.
研究的目的:
- 设计和合成新的深红色光探针,以准线粒体.
- 与MTDR相比,评估它们的光稳定性,细胞吸收,毒性和准效率.
主要方法:
- 一系列 meso-pyridinium BODIPY 染料的合成,具有不同的脂性头部部分和替代.
- 光物理性质的表征 (吸收,发射,斯托克斯转移).
- 在活细胞中评估细胞吸收,线粒体定位,光稳定性和细胞毒性.
主要成果:
- 合成了具有长时间吸收和良好的排放的新型BODY染料.
- 带有基头和糖醇替代 (3h) 的中正正金BODIPY显示出优异的线粒体向,高光稳定性,低毒性和良好的细胞吸收.
- 一种不移动的衍生品 (3i) 在受损的线粒体膜潜力下保持了准有效性.
结论:
- BODIPY衍生物3h和3i是MTDR用于长波长线粒体成像的有希望的替代品.
- 这些探针适用于长期的线粒体跟踪和在具有挑战性的细胞条件下的研究.
相关概念视频
UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Gas Chromatography: Types of Detectors-II
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...


