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在瑞克塞病毒感染期间,识别出在选择性包装的外体病原性微RNA中独占共享的常见序列动图

Jiani Bei¹, Yuan Qiu¹, Diane Cockrell²

¹Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.

Journal of cellular physiology

|June 19, 2023

概括

感染瑞凯菌的内皮细胞外体 (R-ECExos) 破坏了微血管内皮细胞屏障. 像miR23a和miR30b这样的特定微RNA (miRNA) 被选择性地丰富在R-ECExos中,导致了这种屏障功能障碍.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
病原体与宿主之间的相互作用

背景情况:

斑点性发烧 (Rickettsiosis) 正在增加,通过影响大脑和肺组织,导致严重的疾病.
之前已经表明,感染瑞凯菌的内皮细胞衍生外体 (R-ECExos) 含有特定的微RNA (miRNA).
在接受微血管内皮细胞 (MECs) 中,R-ECExos诱导屏障功能障碍的机制尚不清楚.

研究的目的:

在接受MECs中研究R-ECExos诱导的屏障功能障碍的分子机制.
确定外体RNA载荷在调解R-ECExos效应中的作用.
识别R-ECExos中丰富的特定miRNA及其潜在的分类机制.

主要方法:

用来自Rickettsia parkeri感染的人类皮肤MECs的R-ECExos治疗接受者的肺MECs (PMECs).
在接受者PMEC中评估VE-cadherin表达和细胞屏障功能.
在母MEC和R-ECExos中量化miRNA水平.
对miRNA序列的生物信息分析以确定共同的动机.

主要成果:

在接受R-ECExos的患者中,R-ECExos治疗干扰了VE-cadherin,并损害了接受PMEC的屏障功能.
屏障功能障碍取决于R-ECExos的外体RNA含量.
与微血管病相关的miR23a-27a-24集群和miR30b被选择性地丰富R-ECExos.
在感染后的母皮MEC中没有观察到miRNA水平的显著差异.
在选择性丰富的外体miRNA中确定了常见的序列动机 (ACA,UCA,CAG).

结论:

R-ECExos可以选择性地丰富特定的miRNA,包括miR23a-27a-24集群和miR30b.
这些丰富的miRNAs有助于R-ECExos诱导的微血管内皮细胞屏障功能障碍.
这些miRNA中的序列动图可能在它们被选择性地分类为外体体中发挥作用.
需要进一步的研究,以阐明miRNA分类的精确机制及其在皮病感染中的功能后果.

关键词:

内皮壁障碍的功能障碍是内皮壁障碍.外基因组是外基因组中的一个.流动性的AFM AFM 这是一个微型RNA.里克塞斯感染里克塞斯感染序列图案是一个序列图案.

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