一个组织内放射学微观探测器,用于在活体组织中测量辐射
Amanda L Holt1, Yakir Luc Gagnon2, Alison M Sweeney3
1Department of Physics, Yale University; Department of Physics and Astronomy, University of Pennsylvania.
Journal of visualized experiments : JoVE
|June 19, 2023
概括
研究人员开发了光学微探测器,以测量生活组织深处的光. 这些探测器揭示了光线透到诸如大脑和巨型等组织中,有助于研究光线的研究.
科学领域:
- 生物光子学和光学传感器
- 在体内光传输和组织光学.
- 微尺度的光学测量方法
背景情况:
- 生物组织散射和吸收光线,使内部光线水平难以测量.
- 像大脑和脂肪这样的组织中的光反应性蛋白质表明光发挥着作用,但它们的功能尚不清楚.
- 了解内部光对于各种环境中的光合作用和生态系统生产力至关重要.
研究的目的:
- 开发一种方法来测量活组织内部的标量辐射和下流辐射.
- 创建光学微探测器以细胞分辨率进行现场光测量.
- 研究复杂的生物系统中的光传播.
主要方法:
- 使用热拉光纤和玻璃管 pipette 制造微探针.
- 附加一个注入二氧化的环氧球,以改变角接受.
- 使用微操纵器在现场插入和精确定位探头.
- 在10-100微米的空间分辨率下测量组织辐射.
主要成果:
- 在4毫米深度到达小鼠脂肪和脑细胞的光的表征.
- 评估藻类丰富的巨组织中的光水平.
- 在实地实验室中展示微型探测器的能力.
结论:
- 开发的光学微探测器可以在现场测量活体组织中的光.
- 这项技术为以前无法进入的生物环境中的光透提供了洞察力.
- 这些发现有助于理解光在组织功能和生物生产力中的作用.
相关概念视频
01:07Scanning Electron Microscopy
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
01:22Overview of Microscopy Techniques
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...