从Bacillus subtilis中通过循环德克斯林葡萄糖转移酶有效地产生α-单一葡萄糖西丁
Jiawei Zhou1,2, Yuan Shi1,2, Jingyi Fang1,2
1Institute of Fermentation Engineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Applied microbiology and biotechnology
|June 19, 2023
概括
研究人员开发了一种安全高效的方法,使用Bacillus subtilis生产alpha-monoglucosyl hesperidin. 这一过程显著增加了酶活性,并实现了这一有价值的食品添加剂的创纪录产量.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么? 酶工程是什么
- 食品科学 食品科学 食品科学
背景情况:
- 阿尔法-单糖 hesperidin 是一种有价值的食品添加剂,具有多种生物活动.
- 目前生产alpha-monoglucosyl hesperidin的方法有限,因此需要改进合成策略.
研究的目的:
- 开发一种用于合成alpha-monoglucosyl hesperidin的实用和安全的工艺.
- 在非致病宿主中优化循环德克斯特林葡萄糖转移酶 (CGTase) 的表达和分泌.
主要方法:
- 使用Bacillus subtilis作为表达来自Bacillus sp.的CGTase的主体. 一个A2-5a.
- 选了各种促进剂和信号来增强CGTase转录和分泌.
- 采用信号的高通量选程序.
主要成果:
- 确定YdjM是最佳的信号和PaprE是最好的促进剂.
- 实现了CGTase酶活性为46.5U mL-1的8.7倍增长.
- 通过酶合成获得的最大产量为2.70g L-1的α-monoglucosyl hesperidin.
结论:
- 建立了一个优化的重组细菌菌株,用于高水平的CGTase生产.
- 证明了一种可扩展和高效的方法来生产alpha-monoglucosyl hesperidin.
- 这项工作为使用重组CGTase的alpha-monoglucosyl hesperidin生产设定了新的基准.
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